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Literature summary for 1.4.1.14 extracted from

  • Cullimore, J.V.; Sims, A.P.
    Occurrence of two forms of glutamate synthase in Chlamydomonas reinhardii (1981), Phytochemistry, 20, 597-600.
No PubMed abstract available
Search for more literature for 1.4.1.14

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Chlamydomonas reinhardtii
high levels of sucrose stabilize Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
azaserine
-
 Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 2-oxoglutarate
-
 Chlamydomonas reinhardtii
0.013
-
 NADH
-
 Chlamydomonas reinhardtii
0.9
-
 L-glutamine
-
 Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
240000
-
 sedimentation studies Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH Chlamydomonas reinhardtii ammonia assimilation glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH Chlamydomonas reinhardtii CCAP 11/32A ammonia assimilation glutamate + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-
Chlamydomonas reinhardtii CCAP 11/32A
-
-
-

Oxidation Stability

Oxidation Stability Organism
sensitive to oxygen Chlamydomonas reinhardtii

Purification (Commentary)

Purification (Comment) Organism
partial purification Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH specific for NADH Chlamydomonas reinhardtii L-glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH specific for NADH Chlamydomonas reinhardtii CCAP 11/32A L-glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH ammonia assimilation Chlamydomonas reinhardtii glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH ammonia assimilation Chlamydomonas reinhardtii CCAP 11/32A glutamate + NAD+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Chlamydomonas reinhardtii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 cold-labile enzyme Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Chlamydomonas reinhardtii
NADH specific for NADH as reductant Chlamydomonas reinhardtii