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Literature summary for 1.4.1.14 extracted from

  • Marquez, A.J.; Galvan, F.; Vega, J.M.
    Purification and characterization of the NADH-glutamate synthase from Chlamydomonas reinhardii (1984), Plant Sci. Lett., 34, 305-314.
No PubMed abstract available
Search for more literature for 1.4.1.14

Inhibitors

Inhibitors Comment Organism Structure
6-diazo-5-oxo-L-norleucine 76% inhibition at 5 mM Chlamydomonas reinhardtii
acridine inhibitor of flavoenzymes, 65% inhibition Chlamydomonas reinhardtii
azaserine 75% inhibition at 5 mM Chlamydomonas reinhardtii
bathophenanthroline 50% inhibition Chlamydomonas reinhardtii
additional information not inhibited by 1 mM cyanide or cyanate Chlamydomonas reinhardtii
p-hydroxymercuribenzoate complete inhibition at 0.1-1 mM, at 0.0005 mM 33% inhibition or 75% inhibition after preincubation in presence of glutamine and 2-oxoglutarate, substrates induce a conformation, which makes essential sulfhydryl groups more accessible to reagent Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Chlamydomonas reinhardtii
0.0076
-
 NADH
-
 Chlamydomonas reinhardtii
0.0182
-
 2-oxoglutarate
-
 Chlamydomonas reinhardtii
0.6
-
 L-glutamine
-
 Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-
Chlamydomonas reinhardtii 21 gr
-
-
-

Oxidation Stability

Oxidation Stability Organism
4°C, a few days under air, stable Chlamydomonas reinhardtii

Purification (Commentary)

Purification (Comment) Organism
350fold purification Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.56
-
-
 Chlamydomonas reinhardtii

Storage Stability

Storage Stability Organism
4°C, a few days under air, stable Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH highly specific for its substrates Chlamydomonas reinhardtii L-glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH specific for NADH Chlamydomonas reinhardtii L-glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH highly specific for its substrates Chlamydomonas reinhardtii 21 gr L-glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH specific for NADH Chlamydomonas reinhardtii 21 gr L-glutamate + NAD+
-
 ?
additional information no activity with hydroxylamine Chlamydomonas reinhardtii ?
-
 ?
additional information no activity with L-asparagine, NH4Cl, pyruvate, oxaloacetate or NADPH Chlamydomonas reinhardtii ?
-
 ?
additional information no activity with hydroxylamine Chlamydomonas reinhardtii 21 gr ?
-
 ?
additional information no activity with L-asparagine, NH4Cl, pyruvate, oxaloacetate or NADPH Chlamydomonas reinhardtii 21 gr ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 10 min, 86% loss of activity Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
NADH specific for NADH as reductant Chlamydomonas reinhardtii