Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparison, quantitative real-time PCR expression analysis, recombinant expression in Escherichia coli strain BL21(DE3) | Entamoeba histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 2[4Fe-4S]-containing enzyme | Entamoeba histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | C4LUA1 | EhNO1 | - |
Entamoeba histolytica | C8KIP3 | EhNO2 | - |
Entamoeba histolytica HM1:IMSS cl 6 | C4LUA1 | EhNO1 | - |
Entamoeba histolytica HM1:IMSS cl 6 | C8KIP3 | EhNO2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | - |
Entamoeba histolytica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the FAD and 2[4Fe-4S]-containing enzyme does not act as glutamate synthase, which is supported by phylogenetic analyses. Rather, it catalyzes the NADPH-dependent reduction of oxygen to hydrogen peroxide and L-cystine to L-cysteine and also function as ferric and ferredoxin-NADP+ reductase. EhNO1 and EhNO2 show notable differences in substrate specificity and catalytic efficiency. EhNO1 has lower Km and higher kcat/Km values for ferric ion and ferredoxin than EhNO2, whereas EhNO2 prefers L-cystine as a substrate. EhNO1 and EhNO2 also reduce metronidazole | Entamoeba histolytica | ? | - |
? | |
additional information | the FAD and 2[4Fe-4S]-containing enzyme does not act as glutamate synthase, which is supported by phylogenetic analyses. Rather, it catalyzes the NADPH-dependent reduction of oxygen to hydrogen peroxide and L-cystine to L-cysteine and also function as ferric and ferredoxin-NADP+ reductase. EhNO1 and EhNO2 show notable differences in substrate specificity and catalytic efficiency. EhNO1 has lower Km and higher kcat/Km values for ferric ion and ferredoxin than EhNO2, whereas EhNO2 prefers L-cystine as a substrate. EhNO1 and EhNO2 also reduce metronidazole | Entamoeba histolytica HM1:IMSS cl 6 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EhNO1 | - |
Entamoeba histolytica |
EhNO2 | - |
Entamoeba histolytica |
GOGAT | - |
Entamoeba histolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Entamoeba histolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Entamoeba histolytica | |
NADPH | - |
Entamoeba histolytica |
General Information | Comment | Organism |
---|---|---|
physiological function | the small subunit-like protein of glutamate synthase might play an important role in redox maintenance, L-cysteine/L-cystine homeostasis, iron reduction, and the activation of metronidazole | Entamoeba histolytica |