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Literature summary for 1.4.1.13 extracted from

  • Carlberg, I.; Norlund, S.
    Purification and partial characterization of glutamate synthase from Rhodospirillum rubrum grown under nitrogen-fixing conditions (1991), Biochem. J., 279, 151-154.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.13

Inhibitors

Inhibitors Comment Organism Structure
6-diazo-5-oxo-L-norleucine 35% inhibition at 0.0025 mM, when reaction is initiated with 0.5 mM L-glutamine, 73% inhibition at 0.0025 mM, when reaction is initiated with 2.5 mM 2-oxoglutarate Rhodospirillum rubrum
azaserine 38% inhibition at 0.0125 mM when reaction is initiated with 0.5 mM L-glutamine and 69% inhibition at 0.0125 mM when reaction is initiated with 2.5 mM 2-oxoglutarate Rhodospirillum rubrum
L-aspartate
-
 Rhodospirillum rubrum
L-methionine
-
 Rhodospirillum rubrum
NADP+ competitive inhibitor with NADPH, 54% inhibition at 1 mM Rhodospirillum rubrum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 2-oxoglutarate
-
 Rhodospirillum rubrum
0.016
-
 NADPH
-
 Rhodospirillum rubrum
0.065
-
 L-glutamine
-
 Rhodospirillum rubrum

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-sulfur protein Rhodospirillum rubrum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 x * 152000 + x * 53000, SDS-PAGE Rhodospirillum rubrum
152000
-
 x * 152000 + x * 53000, SDS-PAGE Rhodospirillum rubrum
800000
-
 gel filtration, presence of Cl- Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADPH Rhodospirillum rubrum main route for assimilation of ammonium compounds L-glutamate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate precipitation and column chromatography on DEAE-Sepharose, 2',5'-ADP-Sepharose and Sephacryl S-300 Rhodospirillum rubrum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21
-
-
 Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADPH main route for assimilation of ammonium compounds Rhodospirillum rubrum L-glutamate + NADP+
-
 ?
L-glutamine + 2-oxoglutarate + NADPH + H+ no activity with NH4+ Rhodospirillum rubrum L-glutamate + NADP+
-
 ?
NH3 + 2-oxoglutarate + NADPH + H+ the activity with 10 mM NH4+ ions is less than 2% that with L-glutamine Rhodospirillum rubrum L-glutamate + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 152000 + x * 53000, SDS-PAGE Rhodospirillum rubrum

Cofactor

Cofactor Comment Organism Structure
flavin flavoenzyme Rhodospirillum rubrum
NADH no activity with NADH Rhodospirillum rubrum
NADPH
-
 Rhodospirillum rubrum