Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Bromo-2-oxoglutarate | - |
Escherichia coli | |
4-Iodoacetamidosalicylate | inactivation is faster at pH 4.6 to 5.5 compared to pH 6.5 to 7.2 | Escherichia coli | |
Bromopyruvate | - |
Escherichia coli | |
iodoacetamide | - |
Escherichia coli | |
p-chloromercuribenzoate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
NADPH | NH3-dependent glutamate synthase and apoglutamate synthase | Escherichia coli | |
0.24 | - |
2-oxoglutarate | NH3-dependent glutamate synthase and apoglutamate synthase | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
0.24 | - |
apoenzyme lacking flavin and non-heme iron, glutamine-dependent activity | Escherichia coli |
0.92 | - |
native enzyme, NH3-dependent activity | Escherichia coli |
4.45 | - |
apoenzyme lacking flavin and non-heme iron, NH3-dependent activity | Escherichia coli |
18.64 | - |
native enzyme, glutamine-dependent activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADPH + H+ | both native and apoglutamate synthase catalyze NADP+ reduction at approximately 12% the rate of NADPH oxidation | Escherichia coli | L-glutamate + NADP+ | - |
r | |
additional information | NH3-dependent activity is increased approximately 5fold in apoglutamate synthase lacking flavin and non-heme iron | Escherichia coli | ? | - |
? | |
NH3 + 2-oxoglutarate + NADPH + H+ | - |
Escherichia coli | L-glutamate + NADP+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
for glutamate synthesis, NH3-dependent glutamate synthase and apoenzyme | Escherichia coli |
9.4 | - |
optimum for glutamate-dependent reduction of NADP+, NH3-dependent enzyme, apoglutamate synthase enzyme | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.86 | - |
p-chloromercuribenzoate | - |
Escherichia coli |