Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxo-L-norleucine | potent inhibitor, complete loss of activity at 0.1 mM after 10 min of preincubation | Azospirillum brasilense | |
CaCl2 | - |
Azospirillum brasilense | |
KBr | - |
Azospirillum brasilense | |
KI | - |
Azospirillum brasilense | |
L-asparagine | activity decreases to about 0.5% of the original at 25 mM | Azospirillum brasilense | |
L-methionine | competitive inhibitor with respect to L-glutamine | Azospirillum brasilense | |
NaClO4 | - |
Azospirillum brasilense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00625 | - |
NADPH | - |
Azospirillum brasilense | |
0.029 | - |
2-oxoglutarate | - |
Azospirillum brasilense | |
0.45 | - |
L-glutamine | - |
Azospirillum brasilense |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron-sulfur protein | Azospirillum brasilense | |
Iron | 8.1 iron atoms per MW 185000 | Azospirillum brasilense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 135000 + 4 * 50000, SDS-PAGE | Azospirillum brasilense |
135000 | - |
4 * 135000 + 4 * 50000, SDS-PAGE | Azospirillum brasilense |
740000 | - |
gel filtration | Azospirillum brasilense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azospirillum brasilense | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using heat treatment, ammonium sulfate precipitation, column chromatography on DEAE-Trisacryl, gel filtration and affinity chromatogray | Azospirillum brasilense |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.5 | - |
- |
Azospirillum brasilense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADPH + H+ | when L-glutamine is replaced by ammonia as the amino-group donor, the catalytic activity is less than 1% | Azospirillum brasilense | L-glutamate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 4 * 135000 + 4 * 50000, SDS-PAGE | Azospirillum brasilense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Azospirillum brasilense |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.3 | 8.3 | - |
Azospirillum brasilense |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavoenzyme | Azospirillum brasilense | |
flavin | contains FAD and FMN | Azospirillum brasilense | |
flavin | ratio FAD: FMN is 1 | Azospirillum brasilense | |
additional information | the enzyme contains 7.9 sulfur atoms per protomer with a molecular weight of 185000 | Azospirillum brasilense | |
NADH | no activity with NADH | Azospirillum brasilense | |
NADPH | - |
Azospirillum brasilense |