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Literature summary for 1.4.1.13 extracted from

  • Ratti, S.; Curti, B.; Zanetti, G.; Galli, E.
    Purification and characterization of glutamate synthase from Azospirillum brasilense (1985), J. Bacteriol., 163, 724-729.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
6-diazo-5-oxo-L-norleucine potent inhibitor, complete loss of activity at 0.1 mM after 10 min of preincubation Azospirillum brasilense
CaCl2
-
Azospirillum brasilense
KBr
-
Azospirillum brasilense
KI
-
Azospirillum brasilense
L-asparagine activity decreases to about 0.5% of the original at 25 mM Azospirillum brasilense
L-methionine competitive inhibitor with respect to L-glutamine Azospirillum brasilense
NaClO4
-
Azospirillum brasilense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00625
-
NADPH
-
Azospirillum brasilense
0.029
-
2-oxoglutarate
-
Azospirillum brasilense
0.45
-
L-glutamine
-
Azospirillum brasilense

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-sulfur protein Azospirillum brasilense
Iron 8.1 iron atoms per MW 185000 Azospirillum brasilense

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
4 * 135000 + 4 * 50000, SDS-PAGE Azospirillum brasilense
135000
-
4 * 135000 + 4 * 50000, SDS-PAGE Azospirillum brasilense
740000
-
gel filtration Azospirillum brasilense

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using heat treatment, ammonium sulfate precipitation, column chromatography on DEAE-Trisacryl, gel filtration and affinity chromatogray Azospirillum brasilense

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.5
-
-
Azospirillum brasilense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADPH + H+ when L-glutamine is replaced by ammonia as the amino-group donor, the catalytic activity is less than 1% Azospirillum brasilense L-glutamate + NADP+
-
?

Subunits

Subunits Comment Organism
octamer 4 * 135000 + 4 * 50000, SDS-PAGE Azospirillum brasilense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Azospirillum brasilense

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.3 8.3
-
Azospirillum brasilense

Cofactor

Cofactor Comment Organism Structure
flavin flavoenzyme Azospirillum brasilense
flavin contains FAD and FMN Azospirillum brasilense
flavin ratio FAD: FMN is 1 Azospirillum brasilense
additional information the enzyme contains 7.9 sulfur atoms per protomer with a molecular weight of 185000 Azospirillum brasilense
NADH no activity with NADH Azospirillum brasilense
NADPH
-
Azospirillum brasilense