Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-4-ketopentanoate | 70% inhibition in the presence of 1 mM 2-amino-4-ketopentanoate | Acetoanaerobium sticklandii | |
acetyl-CoA | 30% inhibition in the presence of 1 mM acetyl-CoA | Acetoanaerobium sticklandii | |
D-alanine | 45% inhibition in the presence of 1 mM D-alanine | Acetoanaerobium sticklandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
NADP+ | - |
Acetoanaerobium sticklandii | |
2100 | - |
NAD+ | - |
Acetoanaerobium sticklandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
SDS-PAGE | Acetoanaerobium sticklandii |
38930 | - |
calculated from cDNA | Acetoanaerobium sticklandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetoanaerobium sticklandii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-diaminopentanoate + H2O + NAD+ | enzyme is more efficient with the natural substrate (2R,4S)-2,4-diaminopentanoate (Vmax: 51.6/sec) it also reacts with (2R,4R)-2,4-diaminopentanoate (Vmax: 2.8/sec) | Acetoanaerobium sticklandii | 2-amino-4-oxopentanoate + NH3 + NADH | - |
? | |
2,4-diaminopentanoate + H2O + NADP+ | enzyme is more efficient with the natural substrate (2R,4S)-2,4-diaminopentanoate (Vmax: 51.6/sec) it also reacts with (2R,4R)-2,4-diaminopentanoate (Vmax: 2.8/sec) | Acetoanaerobium sticklandii | 2-amino-4-oxopentanoate + NH3 + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | gel filtration, 2 * 38000 Da | Acetoanaerobium sticklandii |
Synonyms | Comment | Organism |
---|---|---|
2R,4S-2,4-diaminopentanoate dehydrogenase | - |
Acetoanaerobium sticklandii |
DAPDH | - |
Acetoanaerobium sticklandii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assay carried out at room temperature | Acetoanaerobium sticklandii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
NADP+ | - |
Acetoanaerobium sticklandii | |
34 | - |
NAD+ | - |
Acetoanaerobium sticklandii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Acetoanaerobium sticklandii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme can function with both nucleotides as hydrogen acceptors, NADP+ and NAD+ | Acetoanaerobium sticklandii | |
NADP+ | the enzyme can function with both nucleotides as hydrogen acceptors, NADP+ and NAD+ | Acetoanaerobium sticklandii | |
pyridoxal 5'-phosphate | - |
Acetoanaerobium sticklandii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
NAD+ | - |
Acetoanaerobium sticklandii | |
2.56 | - |
NADP+ | - |
Acetoanaerobium sticklandii |