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Literature summary for 1.4.1.1 extracted from

  • Bae, J.D.; Cho, Y.J.; Kim, D.I.; Lee, D.S.; Shin, H.J.
    Purification and biochemical characterization of recombinant alanine dehydrogenase from Thermus caldophilus GK24 (2003), J. Microbiol. Biotechnol., 13, 628-631.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
CHCl3 20% Thermus caldophilus
hexane 20% Thermus caldophilus
Zn2+ 1 mM Thermus caldophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
pyruvate
-
Thermus caldophilus
1.8
-
NAD+
-
Thermus caldophilus
2.6
-
L-Ala
-
Thermus caldophilus
39.4
-
NH4+
-
Thermus caldophilus
50
-
NADH
-
Thermus caldophilus

Organism

Organism UniProt Comment Textmining
Thermus caldophilus
-
-
-
Thermus caldophilus GK24
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thermus caldophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala + H2O + NAD+
-
Thermus caldophilus pyruvate + NH3 + NADH
-
r
L-Ala + H2O + NAD+
-
Thermus caldophilus GK24 pyruvate + NH3 + NADH
-
r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
stable Thermus caldophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
reductive amination of pyruvate Thermus caldophilus
12
-
oxidative deamination of L-Ala Thermus caldophilus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Thermus caldophilus
NADH
-
Thermus caldophilus