Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CHCl3 | 20% | Thermus caldophilus | |
hexane | 20% | Thermus caldophilus | |
Zn2+ | 1 mM | Thermus caldophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
pyruvate | - |
Thermus caldophilus | |
1.8 | - |
NAD+ | - |
Thermus caldophilus | |
2.6 | - |
L-Ala | - |
Thermus caldophilus | |
39.4 | - |
NH4+ | - |
Thermus caldophilus | |
50 | - |
NADH | - |
Thermus caldophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus caldophilus | - |
- |
- |
Thermus caldophilus GK24 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Thermus caldophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala + H2O + NAD+ | - |
Thermus caldophilus | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Thermus caldophilus GK24 | pyruvate + NH3 + NADH | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable | Thermus caldophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reductive amination of pyruvate | Thermus caldophilus |
12 | - |
oxidative deamination of L-Ala | Thermus caldophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermus caldophilus | |
NADH | - |
Thermus caldophilus |