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Literature summary for 1.4.1.1 extracted from

  • Smith, M.T.; Emerich, D.W.
    Alanine dehydrogenase from soybean nodule bacteroids: purification and properties (1993), Arch. Biochem. Biophys., 304, 379-385.
    View publication on PubMed
Search for more literature for 1.4.1.1

Inhibitors

Inhibitors Comment Organism Structure
L-Ala substrate inhibition Rhizobium sp.
methylglyoxal
-
 Rhizobium sp.
NADH product inhibition Rhizobium sp.
NH4+
-
 Rhizobium sp.
oxamate
-
 Rhizobium sp.
propionate
-
 Rhizobium sp.
pyruvate substrate inhibition Rhizobium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 NADH
-
 Rhizobium sp.
0.098
-
 NADH
-
 Rhizobium sp.
0.37
-
 L-Ala
-
 Rhizobium sp.
0.43
-
 pyruvate
-
 Rhizobium sp.
5.5
-
 NH4+
-
 Rhizobium sp.

Organism

Organism UniProt Comment Textmining
Rhizobium sp.
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH + H+ the kinetic mechanism at pH 8.5 is determined to be ter-bi Theorell-Chance. In the amination direction, the substrates add in the order: NADH, NH4+, pyruvate, with NH4+ binding in rapid-equilibrium. In the reverse direction, NAD adds first followed by L-Ala Rhizobium sp.
a

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala + H2O + NAD+
-
 Rhizobium sp. pyruvate + NH3 + NADH
-
 r

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Rhizobium sp.
NADH
-
 Rhizobium sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.092
-
 pyruvate
-
 Rhizobium sp.
1
-
 L-Ala
-
 Rhizobium sp.
3.7
-
 NH4+
-
 Rhizobium sp.