General Stability | Organism |
---|---|
repeated freezing and thawing leads to significant loss of activity | Bilophila wadsworthia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
NADH | - |
Bilophila wadsworthia | |
0.15 | - |
NAD+ | - |
Bilophila wadsworthia | |
1.1 | - |
pyruvate | - |
Bilophila wadsworthia | |
1.6 | - |
L-Ala | - |
Bilophila wadsworthia | |
31 | - |
NH4+ | - |
Bilophila wadsworthia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39900 | - |
6 * 39900, calculation from nucleotide sequence | Bilophila wadsworthia |
42000 | - |
6 * 42000, SDS-PAGE | Bilophila wadsworthia |
273000 | - |
gel filtration | Bilophila wadsworthia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bilophila wadsworthia | enzyme is involved in taurine metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bilophila wadsworthia | Q9AIK2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bilophila wadsworthia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bilophila wadsworthia |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 1 month | Bilophila wadsworthia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ala + H2O + NAD+ | 3.5% of the activity with L-Ala | Bilophila wadsworthia | pyruvate + NH3 + NADH | - |
? | |
D-Ser + H2O + NAD+ | 4.4% of the activity with L-Ala | Bilophila wadsworthia | 3-hydroxypyruvate + NH3 + NADH | - |
r | |
D-Ser + H2O + NAD+ | in the reverse reaction the enzyme reacts at 9.3% of the activity with pyruvate | Bilophila wadsworthia | 3-hydroxypyruvate + NH3 + NADH | - |
r | |
glyoxylate + NH3 + NADH | - |
Bilophila wadsworthia | aminoacetate + NAD+ + H2O | - |
? | |
L-2-aminobutanoate + H2O + NAD+ | in the reverse reaction the enzyme reacts at 15.3% of the activity with pyruvate | Bilophila wadsworthia | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | 10.4% of the activity with L-Ala | Bilophila wadsworthia | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-Ala + H2O + NAD+ | - |
Bilophila wadsworthia | pyruvate + NH3 + NADH | - |
r | |
L-Ile + H2O + NAD+ | - |
Bilophila wadsworthia | 2-oxo-3-methylpentanoate + NH3 + NADH | - |
? | |
L-norvaline + H2O + NAD+ | at 4.6% of the activity with L-Ala | Bilophila wadsworthia | 2-oxopentanoate + NH3 + NADH | - |
? | |
L-Val + H2O + NAD+ | 4.8% of the activity with L-Ala | Bilophila wadsworthia | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
additional information | no activity with taurine | Bilophila wadsworthia | ? | - |
? | |
additional information | enzyme is involved in taurine metabolism | Bilophila wadsworthia | ? | - |
? | |
oxaloacetate + NH3 + NADH | - |
Bilophila wadsworthia | L-Asp + H2O + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 42000, SDS-PAGE | Bilophila wadsworthia |
hexamer | 6 * 39900, calculation from nucleotide sequence | Bilophila wadsworthia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | oxidative deamination | Bilophila wadsworthia |
55 | 60 | reductive amination | Bilophila wadsworthia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
inactivation after about 1 min | Bilophila wadsworthia |
65 | - |
inactivation after about 10 s | Bilophila wadsworthia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
reductive amination of pyruvate | Bilophila wadsworthia |
9 | 11.5 | oxidative deamination of L-Ala | Bilophila wadsworthia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bilophila wadsworthia | |
NADH | - |
Bilophila wadsworthia |