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Literature summary for 1.4.1.1 extracted from
- Alanine dehydrogenase of the N2-fixing blue-green alga, Anbabaena cylindrica (1976), Arch. Microbiol., 107, 115-124.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Borate | - |
Anabaena cylindrica |  |
| glycine | - |
Anabaena cylindrica | |
| L-Ala | - |
Anabaena cylindrica | |
| L-Cys | - |
Anabaena cylindrica | |
| L-Ser | - |
Anabaena cylindrica | |
| PCMB | - |
Anabaena cylindrica | |
| Zn2+ | - |
Anabaena cylindrica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
pyruvate | - |
Anabaena cylindrica | |
| 0.4 | - |
L-Ala | - |
Anabaena cylindrica | |
| 3 | - |
oxaloacetate | - |
Anabaena cylindrica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
6 * 43000, SDS-PAGE | Anabaena cylindrica |
| 270000 | - |
gel filtration | Anabaena cylindrica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anabaena cylindrica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Anabaena cylindrica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heterocyst | - |
Anabaena cylindrica | - |
| vegetative | - |
Anabaena cylindrica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 38 | - |
- |
Anabaena cylindrica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Ala + H2O + NAD+ | - |
Anabaena cylindrica | pyruvate + NH3 + NADH | - |
r | |
| L-norvaline + H2O + NAD+ | - |
Anabaena cylindrica | 2-oxopentanoate + NH3 + NADH | - |
? | |
| oxaloacetate + NH3 + NADH | - |
Anabaena cylindrica | L-Asp + H2O + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 43000, SDS-PAGE | Anabaena cylindrica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
reductive amination | Anabaena cylindrica |
| 9.6 | - |
oxidative deamination | Anabaena cylindrica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8.8 | pH 6.8: about 50% of maximal activity, pH 8.8: about 60% of maximal activity, reductive amination | Anabaena cylindrica |
| 8.5 | 10.6 | pH 8.5: about 60% of maximal activity, pH 10.6: about 40% of maximal activity, oxidative deamination | Anabaena cylindrica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Anabaena cylindrica | |
| NADH | - |
Anabaena cylindrica | |
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Release 2023.1 (January 2023)
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