Crystallization (Comment) | Organism |
---|---|
- |
Lysinibacillus sphaericus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Lysinibacillus sphaericus | |
HgCl2 | - |
Lysinibacillus sphaericus | |
Pb2+ | - |
Lysinibacillus sphaericus | |
PCMB | - |
Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
L-norvaline | - |
Lysinibacillus sphaericus | |
0.018 | - |
L-Ala | - |
Lysinibacillus sphaericus | |
0.02 | - |
L-Val | - |
Lysinibacillus sphaericus | |
0.039 | - |
L-Ser | - |
Lysinibacillus sphaericus | |
0.33 | - |
L-2-aminobutanoate | - |
Lysinibacillus sphaericus | |
1.7 | - |
pyruvate | - |
Lysinibacillus sphaericus | |
11 | - |
2-oxobutanoate | - |
Lysinibacillus sphaericus | |
11 | - |
2-oxo-3-methylbutanoate | - |
Lysinibacillus sphaericus | |
12 | - |
glyoxylate | - |
Lysinibacillus sphaericus | |
23 | - |
2-oxopentanoate | - |
Lysinibacillus sphaericus | |
80 | - |
3-hydroxypyruvate | - |
Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
230000 | - |
gel filtration | Lysinibacillus sphaericus |
231000 | - |
equilibrium sedimentation | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lysinibacillus sphaericus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH + H+ | sequential ordered ternary-binary mechanism, the enzyme is A-stereospecific, the pro-R hydrogen at C-4 of the reduced nicotinamide ring of NADH is exclusively transferred to pyruvate | Lysinibacillus sphaericus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
157 | - |
- |
Lysinibacillus sphaericus |
Storage Stability | Organism |
---|---|
4°C, 80% saturated ammonium sulfate suspension, pH 7-8, 2 years, stable | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + NH3 + NADH | - |
Lysinibacillus sphaericus | L-Val + NAD+ + H2O | - |
? | |
2-oxobutanoate + NH3 + NADH | - |
Lysinibacillus sphaericus | 2-aminobutanoate + H2O + NAD+ | - |
r | |
glyoxylate + NH3 + NADH | - |
Lysinibacillus sphaericus | aminoacetate + NAD+ + H2O | - |
? | |
L-2-aminobutanoate + H2O + NAD+ | - |
Lysinibacillus sphaericus | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-Ala + H2O + NAD+ | - |
Lysinibacillus sphaericus | pyruvate + NH3 + NADH | - |
r | |
L-norvaline + H2O + NAD+ | - |
Lysinibacillus sphaericus | 2-oxopentanoate + NH3 + NADH | - |
r | |
L-Ser + H2O + NAD+ | - |
Lysinibacillus sphaericus | 3-hydroxypyruvate + NH3 + NADH | - |
r | |
L-Val + H2O + NAD+ | - |
Lysinibacillus sphaericus | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
increase of activity up to | Lysinibacillus sphaericus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 6.0-10.0, 5 min, stable | Lysinibacillus sphaericus |
60 | - |
pH 7.2, 10 mM potassium phosphate buffer, 5 min, stable up to | Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
reductive amination | Lysinibacillus sphaericus |
10 | 10.5 | oxidative deamination | Lysinibacillus sphaericus |
10.5 | - |
deamination of L-Ala | Lysinibacillus sphaericus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | 50°C, 5 min, stable | Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lysinibacillus sphaericus | |
NADH | - |
Lysinibacillus sphaericus |