General Stability | Organism |
---|---|
urea, above 3 M, denaturation of soluble enzyme, resistance to denaturation increases by immobilization, 5 M, 30 min, 85% loss of activity of matrix-bound enzyme | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
NADH | soluble enzyme | Bacillus cereus | |
0.18 | - |
NAD+ | soluble enzyme | Bacillus cereus | |
0.32 | - |
NADH | Sepharose-bound enzyme | Bacillus cereus | |
0.48 | - |
pyruvate | soluble enzyme | Bacillus cereus | |
0.52 | - |
pyruvate | Sepharose-bound enzyme | Bacillus cereus | |
1.18 | - |
NAD+ | Sepharose-bound enzyme | Bacillus cereus | |
11.3 | - |
L-Ala | Sepharose-bound enzyme | Bacillus cereus | |
12.5 | - |
L-Ala | soluble enzyme | Bacillus cereus | |
22 | - |
NH4+ | Sepharose-bound enzyme | Bacillus cereus | |
30 | - |
NH4+ | soluble enzyme | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala + H2O + NAD+ | - |
Bacillus cereus | pyruvate + NH3 + NADH | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
28 d, immobilized enzyme retains 85% of its activity in 0.1 M phosphate buffer, pH 8 | Bacillus cereus |
59 | - |
10 min, 50% loss of activity of soluble enzyme | Bacillus cereus |
65 | - |
10 min, complete loss of activity, soluble enzyme | Bacillus cereus |
67 | - |
10 min, 50% loss of activity, immobilized enzyme | Bacillus cereus |
75 | - |
10 min, complete loss of activity, immobilized enzyme | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | amination reaction, soluble and Sepharose-bound enzyme | Bacillus cereus |
10.5 | 11 | - |
Bacillus cereus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 25°C, 24 h, concentration 0.001 mg/ml, stable | Bacillus cereus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus cereus | |
NADH | - |
Bacillus cereus |