Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activation | Phormidium lapideum |
General Stability | Organism |
---|---|
partial inactivation by dilution | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Phormidium lapideum | |
Cibacron blue | - |
Phormidium lapideum | |
Cu2+ | - |
Phormidium lapideum | |
Hg2+ | - |
Phormidium lapideum | |
L-Ala | - |
Phormidium lapideum | |
L-Phe | reductive amination of pyruvate, 10 mM, 28% inhibition | Phormidium lapideum | |
L-Ser | reductive amination of pyruvate | Phormidium lapideum | |
L-Thr | reductive amination of pyruvate, 10 mM, 37% inhibition | Phormidium lapideum | |
L-Trp | reductive amination of pyruvate, 10 mM, 29% inhibition | Phormidium lapideum | |
PCMB | - |
Bacillus subtilis | |
PCMB | - |
Phormidium lapideum | |
pyridoxal 5'-phosphate | - |
Phormidium lapideum | |
Zn2+ | - |
Bacillus subtilis | |
Zn2+ | - |
Phormidium lapideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADH | - |
Phormidium lapideum | |
0.04 | - |
NAD+ | - |
Phormidium lapideum | |
0.33 | - |
pyruvate | - |
Phormidium lapideum | |
5 | - |
L-Ala | - |
Phormidium lapideum | |
111 | - |
NH4+ | - |
Phormidium lapideum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
6 * 41000, SDS-PAGE | Phormidium lapideum |
240000 | - |
gel filtration | Phormidium lapideum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Phormidium lapideum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phormidium lapideum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
183 | - |
- |
Phormidium lapideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH | 4.1% of the activity with pyruvate | Phormidium lapideum | 2-aminobutanoate + H2O + NAD+ | - |
? | |
3-hydroxypyruvate + NH3 + NADH | 15.4% of the activity with pyruvate | Phormidium lapideum | Ser + H2O + NAD+ | - |
? | |
glyoxylate + NH3 + NADH | 1.2% of the activity with pyruvate | Phormidium lapideum | aminoacetate + NAD+ + H2O | - |
? | |
L-Ala + H2O + NAD+ | - |
Bacillus subtilis | pyruvate + NH3 + NADH | - |
r | |
L-Ala + H2O + NAD+ | - |
Phormidium lapideum | pyruvate + NH3 + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Bacillus subtilis |
hexamer | 6 * 41000, SDS-PAGE | Phormidium lapideum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | reductive amination | Phormidium lapideum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
5 min, pH 6.0-9.0, stable | Phormidium lapideum |
60 | - |
10 min, in presence of 0.3 mM NADH, 30% loss of activity | Phormidium lapideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
reductive amination | Phormidium lapideum |
9.2 | - |
oxidative deamination | Phormidium lapideum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9.2 | pH 7.2: about 40% of maximal activity, pH 9.2: 40% of maximal activity, reductive amination of pyruvate | Phormidium lapideum |
7.8 | 10 | pH 7.8: about 50% of maximal activity, pH 10.0: about 65% of maximal activity, oxidative deamination of L-Ala | Phormidium lapideum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 50°C, 5 min, stable | Phormidium lapideum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis | |
NAD+ | - |
Phormidium lapideum | |
NADH | - |
Bacillus subtilis | |
NADH | - |
Phormidium lapideum |