General Stability | Organism |
---|---|
SDS, 10 min, 37°C, retains activity with up to 0.03% SDS, loss of activity with 0.05% | Lysinibacillus sphaericus |
stable to repeated freezing and thawing | Lysinibacillus sphaericus |
Triton X-100, 10 min, 37°C, not inactivated by treatment with up to 4% | Lysinibacillus sphaericus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuCl2 | - |
Lysinibacillus sphaericus | |
HgCl2 | - |
Lysinibacillus sphaericus | |
NADH | product inhibition | Lysinibacillus sphaericus | |
NH4+ | product inhibition | Lysinibacillus sphaericus | |
PCMB | - |
Lysinibacillus sphaericus | |
pyruvate | product inhibition | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NADH | - |
Lysinibacillus sphaericus | |
0.26 | - |
NAD+ | - |
Lysinibacillus sphaericus | |
0.5 | - |
pyruvate | - |
Lysinibacillus sphaericus | |
10.5 | - |
L-alanine | - |
Lysinibacillus sphaericus | |
38 | - |
NH4+ | - |
Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
6 * 38000, SDS-PAGE | Lysinibacillus sphaericus |
230000 | - |
gel filtration | Lysinibacillus sphaericus |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | 10 min, 37°C, about 90% of activity remains after incubation with up to 35% ethanol, 50% activity with 48% ethanol | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus sphaericus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lysinibacillus sphaericus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH + H+ | reductive amination proceeds through a sequential mechanism containing partially random binding. NADH binds first to the enzyme, and then pyruvate and ammonia bind in a random fashion | Lysinibacillus sphaericus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.4 | - |
- |
Lysinibacillus sphaericus |
Storage Stability | Organism |
---|---|
-20°C, pH 7.2, more than 5 months | Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH | 62% of the activity with pyruvate | Lysinibacillus sphaericus | 2-aminobutanoate + H2O + NAD+ | - |
? | |
3-fluoropyruvate + NH3 + NADH | 97% of the activity with pyruvate | Lysinibacillus sphaericus | 3-fluoro-L-alanine + H2O + NAD+ | - |
? | |
3-hydroxypyruvate + NH3 + NADH | 123% of the activity with pyruvate | Lysinibacillus sphaericus | L-Ser + H2O + NAD+ | - |
? | |
L-2-aminobutanoate + H2O + NAD+ | 2.4% of the activity with L-Ala | Lysinibacillus sphaericus | 2-oxobutanoate + NH3 + NADH + H+ | - |
? | |
L-Ala + H2O + NAD+ | the reaction rate for deamination of pyruvate at the optimum is about 2.8times higher than that for amination of L-Ala at the optimum | Lysinibacillus sphaericus | pyruvate + NH3 + NADH | - |
r | |
oxaloacetate + NH3 + NADH | 99% of the activity with pyruvate | Lysinibacillus sphaericus | L-Asp + H2O + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 38000, SDS-PAGE | Lysinibacillus sphaericus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pH 2.3-10.6, 10 min, stable | Lysinibacillus sphaericus |
75 | - |
60 min, pH 5.5-9.5, stable | Lysinibacillus sphaericus |
80 | - |
20 min, stable | Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
amination of pyruvate | Lysinibacillus sphaericus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | pH 7.0: about 50% of maximal activity, pH 10.0: about 50% of maximal activity, oxidative deamination of L-Ala | Lysinibacillus sphaericus |
8 | 11 | pH 8.0: about 50% of maximal activity, pH 11.0: about 95% of maximal activity, reductive amination of pyruvate | Lysinibacillus sphaericus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.3 | 10.6 | 37°C, 10 min, stable | Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lysinibacillus sphaericus | |
NADH | - |
Lysinibacillus sphaericus |