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Literature summary for 1.3.99.6 extracted from

  • Stokes, N.A.; Hylemon, P.B.
    Characterization of DELTA4-3-ketosteroid-5beta-reductase and 3beta-hydroxysteroid dehydrogenase in cell extracts of Clostridium innocuum (1985), Biochim. Biophys. Acta, 836, 255-261.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
FMNH2 slightly stimulatory in combination with NADH [Clostridium] innocuum
FMNH2 slightly stimulatory in combination with NADH Clostridium paraputrificum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
4-pregnen-3,20-dione
-
[Clostridium] innocuum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
gel filtration [Clostridium] innocuum

Organism

Organism UniProt Comment Textmining
Clostridium paraputrificum
-
-
-
[Clostridium] innocuum
-
-
-

Oxidation Stability

Oxidation Stability Organism
oxygen-sensitive [Clostridium] innocuum
oxygen-sensitive Clostridium paraputrificum

Storage Stability

Storage Stability Organism
-70°C, 20% glycerol, 1 week, less than 30% loss of activity [Clostridium] innocuum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-pregnen-3,20-dione + NADH
-
Clostridium paraputrificum 5beta-pregnan-3,20-dione + NAD+
-
?
4-pregnen-3,20-dione + NADH i.e. progesterone [Clostridium] innocuum 5beta-pregnan-3,20-dione + NAD+
-
?
testosterone + NADH
-
[Clostridium] innocuum 17beta-hydroxy-5beta-androstan-3-one + NAD+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
[Clostridium] innocuum

Cofactor

Cofactor Comment Organism Structure
NADH required [Clostridium] innocuum
NADH required Clostridium paraputrificum