Literature summary for 1.3.99.41 extracted from

Shao, X.; Cao, H.; Zhao, F.; Peng, M.; Wang, P.; Li, C.; Shi, W.; Wei, T.; Yuan, Z.; Zhang, X.; Chen, X.; Todd, J.; Zhang, Y.
Mechanistic insight into 3-methylmercaptopropionate metabolism and kinetical regulation of demethylation pathway in marine dimethylsulfoniopropionate-catabolizing bacteria (2019), Mol. Microbiol., 111, 1057-1073 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.2 M sodium nitrate, 0.1 M Bis-Tris propane (pH 8.5) and 20% (w/v) PEG 3350	Roseovarius nubinhibens

Protein Variants

Protein Variants	Comment	Organism
E435A	the mutation completely abolishes the activity of the enzyme	Roseovarius nubinhibens
F195A	the mutation almost completely abolishes the activity of the enzyme to 0.4% of wild type activity	Roseovarius nubinhibens
F287A	the mutant with 76.3% of wild type activity shows significantly increased Km to 3-(methylsulfanyl)propanoyl-CoA	Roseovarius nubinhibens
H280A	the mutation severely reduces the activity of the enzyme to 18.2%, but does not affect its Km value to 3-(methylsulfanyl)propanoyl-CoA	Roseovarius nubinhibens
K223A	the mutation severely reduces the activity of the enzyme to 9.4%, but does not affect its Km value to 3-(methylsulfanyl)propanoyl-CoA	Roseovarius nubinhibens
K281A	the mutation severely reduces the activity of the enzyme to 54.5%, but does not affect its Km value to 3-(methylsulfanyl)propanoyl-CoA	Roseovarius nubinhibens
M161A	the mutation significantly decreases the activity of the enzyme to 37.5% and does not affect its Km value to FAD or 3-(methylsulfanyl)propanoyl-CoA	Roseovarius nubinhibens
R284A	the mutant with 97.2% of wild type activity shows significantly increased Km to 3-(methylsulfanyl)propanoyl-CoA	Roseovarius nubinhibens
R448A	the mutant with 44.9% of wild type activity shows significantly increased Km for 3-(methylsulfanyl)propanoyl-CoA	Roseovarius nubinhibens
S197A	the mutation significantly decreases the activity of the enzyme to 3.6% and increases its Km value to FAD	Roseovarius nubinhibens
T170A	the mutation significantly decreases the activity of the enzyme to 37.5% and increases its Km value to FAD	Roseovarius nubinhibens
Y434A	the mutation significantly decreases the activity of the enzyme to 51.2% and increases its Km value to FAD but does not affect its Km value to 3-(methylsulfanyl)propanoyl-CoA	Roseovarius nubinhibens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0063	-	FAD	wild type enzyme, at pH 9.0 and 40°C	Roseovarius nubinhibens
0.0092	-	FAD	mutant enzyme M161A, at pH 9.0 and 40°C	Roseovarius nubinhibens
0.0299	-	FAD	mutant enzyme Y434A, at pH 9.0 and 40°C	Roseovarius nubinhibens
0.0648	-	FAD	mutant enzyme S197A, at pH 9.0 and 40°C	Roseovarius nubinhibens
0.1125	-	FAD	mutant enzyme T170A, at pH 9.0 and 40°C	Roseovarius nubinhibens
1.4	-	3-(methylsulfanyl)propanoyl-CoA	wild type enzyme, at pH 9.0 and 40°C	Roseovarius nubinhibens
1.6	-	3-(methylsulfanyl)propanoyl-CoA	mutant enzyme Y434A, at pH 9.0 and 40°C	Roseovarius nubinhibens
2	-	3-(methylsulfanyl)propanoyl-CoA	mutant enzyme H280A, at pH 9.0 and 40°C	Roseovarius nubinhibens
2	-	3-(methylsulfanyl)propanoyl-CoA	mutant enzyme K223A, at pH 9.0 and 40°C	Roseovarius nubinhibens
6.3	-	3-(methylsulfanyl)propanoyl-CoA	mutant enzyme M161A, at pH 9.0 and 40°C	Roseovarius nubinhibens
8	-	3-(methylsulfanyl)propanoyl-CoA	mutant enzyme K281A, at pH 9.0 and 40°C	Roseovarius nubinhibens
12.4	-	3-(methylsulfanyl)propanoyl-CoA	mutant enzyme R284A, at pH 9.0 and 40°C	Roseovarius nubinhibens
14.3	-	3-(methylsulfanyl)propanoyl-CoA	mutant enzyme F287A, at pH 9.0 and 40°C	Roseovarius nubinhibens
100	-	3-(methylsulfanyl)propanoyl-CoA	Km above 100 mM, mutant enzyme R448A, at pH 9.0 and 40°C	Roseovarius nubinhibens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3-(methylsulfanyl)propanoyl-CoA + FAD	Roseovarius nubinhibens	-	3-(methylsulfanyl)acryloyl-CoA + FADH2	-	?

Organism

Organism	UniProt	Comment	Textmining
Roseovarius nubinhibens	A3SI50	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-(methylsulfanyl)propanoyl-CoA + FAD	-	Roseovarius nubinhibens	3-(methylsulfanyl)acryloyl-CoA + FADH2	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 64210, calculated from amino acid sequence	Roseovarius nubinhibens

Synonyms

Synonyms	Comment	Organism
dmdC	-	Roseovarius nubinhibens
MMPA-CoA dehydrogenase	-	Roseovarius nubinhibens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Roseovarius nubinhibens

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	50	more than 70% activity between 30 and 50°C	Roseovarius nubinhibens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Roseovarius nubinhibens

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	9.5	more than 60% activity between pH 5.0 and 9.5	Roseovarius nubinhibens

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Roseovarius nubinhibens

Expression

Organism	Comment	Expression
Roseovarius nubinhibens	the enzyme is up-regulated by dimethylsulfoniopropionate	up

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Release 2023.1 (January 2023)