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Literature summary for 1.3.99.4 extracted from

  • Zhang, W.; Shao, M.; Rao, Z.; Xu, M.; Zhang, X.; Yang, T.; Li, H.; Xu, Z.
    Bioconversion of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione by recombinant Bacillus subtilis expressing ksdd gene encoding 3-ketosteroid-DELTA1-dehydrogenase from Mycobacterium neoaurum JC-12 (2013), J. Steroid Biochem. Mol. Biol., 135, 36-42.
    View publication on PubMed
Search for more literature for 1.3.99.4

Cloned(Commentary)

Cloned (Comment) Organism
gene ksdD, phylogenetic analysis, expression in Bacillus subtilis strain 168 with 18fold higher activity compared to wild-type Mycobacterium neoaurum strain JC-12 Mycolicibacterium neoaurum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-androstene-3,17-dione + FAD Mycolicibacterium neoaurum
-
 1,4-androstadiene-3,17-dione + FADH2
-
 ?
4-androstene-3,17-dione + FAD Mycolicibacterium neoaurum JC-12
-
 1,4-androstadiene-3,17-dione + FADH2
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium neoaurum
-
 gene ksdD
-
Mycolicibacterium neoaurum JC-12
-
 gene ksdD
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.75
-
 recombinant enzyme expressed in Bacillus subtilis, pH 7.0, 30°C Mycolicibacterium neoaurum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-androstene-3,17-dione + FAD
-
 Mycolicibacterium neoaurum 1,4-androstadiene-3,17-dione + FADH2
-
 ?
4-androstene-3,17-dione + FAD with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol Mycolicibacterium neoaurum 1,4-androstadiene-3,17-dione + FADH2
-
 ?
4-androstene-3,17-dione + FAD
-
 Mycolicibacterium neoaurum JC-12 1,4-androstadiene-3,17-dione + FADH2
-
 ?
4-androstene-3,17-dione + FAD with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol Mycolicibacterium neoaurum JC-12 1,4-androstadiene-3,17-dione + FADH2
-
 ?

Synonyms

Synonyms Comment Organism
3-ketosteroid-DELTA1-dehydrogenase
-
 Mycolicibacterium neoaurum
KsdD
-
 Mycolicibacterium neoaurum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mycolicibacterium neoaurum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Mycolicibacterium neoaurum

Cofactor

Cofactor Comment Organism Structure
FAD putative N-terminal FAD-binding motif in KsdDM is GSG(A/G)(A/G)(A/G)X17E Mycolicibacterium neoaurum

General Information

General Information Comment Organism
metabolism the 3-ketosteroid-DELTA1-dehydrogenase is involved in steroid metabolism catalyzing the transformation of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione specifically Mycolicibacterium neoaurum