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Literature summary for 1.3.99.4 extracted from

  • Itagaki, E.; Wakabayashi, T.; Hatta, T.
    Purification and characterization of 3-ketosteroid-delta 1-dehydrogenase from Nocardia corallina (1990), Biochim. Biophys. Acta, 1038, 60-67.
    View publication on PubMed
Search for more literature for 1.3.99.4

Inhibitors

Inhibitors Comment Organism Structure
1-Androstene-3,17-dione
-
 Gordonia rubripertincta
estrone
-
 Gordonia rubripertincta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0063
-
 11beta-hydroxytestosterone i.e. 4-androstene-11beta,17beta-diol-3-one Gordonia rubripertincta
0.0064
-
 11-deoxy-17alpha-hydroxycorticosterone i.e. 4-pregnene-17alpha,21-diol-3,20-dione Gordonia rubripertincta
0.0066
-
 androsterone i.e. 4-androstene-3,11,17-trione Gordonia rubripertincta
0.0083
-
 19-nortestosterone 4-estren-17beta-ol-3one Gordonia rubripertincta
0.0089
-
 19-nor-4-androstene-3,17-dione i.e. 4-estren-3,17-dione Gordonia rubripertincta
0.0143
-
 testololactone i.e. 17alpha-oxo-D-homo-1,4-androstadiene-3,17-dione Gordonia rubripertincta
0.0196
-
 11alpha-hydroxyprogesterone i.e. 4-pregnene-11alpha-ol-3,20-dione, 11beta-hydroxy-4-androstene-3,17-dione, 4-estren-3,17-dione Gordonia rubripertincta
0.0204
-
 corticosterone i.e. 4-pregnene-11beta,21-diol-3,20-dione Gordonia rubripertincta
0.021
-
 4-androstene-3,17-dione
-
 Gordonia rubripertincta
0.0256
-
 cortisone i.e. 4-pregnene-17alpha,21diol-3,11,20-trione Gordonia rubripertincta
0.0385
-
 hydrocorticosterone i.e. 4-pregnene-11beta,17alpha,21-triol-3,20-dione Gordonia rubripertincta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60500
-
 SDS-PAGE, gel filtration Gordonia rubripertincta

Organism

Organism UniProt Comment Textmining
Gordonia rubripertincta
-
 inducible
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gordonia rubripertincta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
113
-
-
 Gordonia rubripertincta

Storage Stability

Storage Stability Organism
4°C, 1 week, stable Gordonia rubripertincta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-deoxy-17alpha-hydroxycorticosterone + acceptor 4-pregnene-17alpha,21-diol-3,20-dione Gordonia rubripertincta 17,21-dihydroxypregna-1,4-diene-3,20-dione + reduced acceptor
-
 ?
11alpha-hydroxyprogesterone + acceptor 4-pregnene-11alpha-ol-3,20-dione Gordonia rubripertincta 11alpha-hydroxy-1,4-pregnene-3,20-dione + reduced acceptor
-
 ?
11beta-hydroxy-4-androstene-3,17-dione + acceptor
-
 Gordonia rubripertincta 11beta-hydroxy-1,4-androstene-3,17-dione + reduced acceptor
-
 ?
11beta-hydroxytestosterone + acceptor 4-androstene-11beta,17beta-diol-3-one Gordonia rubripertincta 11beta,17beta-dihydroxyandrost-1,4-diene-3-one + reduced acceptor
-
 ?
19-nor-4-androstene-3,17-dione + acceptor 4-estren-3,17-dione Gordonia rubripertincta 19-nor-1,4-androstene-3,17-dione + reduced acceptor
-
 ?
19-nortestosterone + acceptor 4-estren-17beta-ol-3-one Gordonia rubripertincta 17beta-hydroxy-1,4-estrene-3-one + reduced acceptor
-
 ?
4-androstene-3,17-dione + acceptor
-
 Gordonia rubripertincta 1,4-androstene-3,17-dione + reduced acceptor
-
 ?
androsterone + acceptor 4-androstene-3,11,17-trione Gordonia rubripertincta 3-alpha-hydroxy-1-androstene-17-one + reduced acceptor
-
 ?
corticosterone + acceptor 4-pregnene-11beta,21-diol-3,20-dione Gordonia rubripertincta 1,4-pregnene-17alpha,21-diol-3,20-dione + reduced acceptor
-
 ?
cortisone + acceptor 4-pregnene-17alpha,21-diol-3,11,20-trione Gordonia rubripertincta 1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor
-
 ?
hydrocorticosterone + acceptor 4-pregnene-11beta,17alpha,21triol-3,20-dione Gordonia rubripertincta 1,4-pregnene-11beta,17alpha,21-triol-3,20-dione + reduced acceptor
-
 ?
additional information acceptor: phenazine methosulfate Gordonia rubripertincta ?
-
 ?
additional information acceptor: O2 Gordonia rubripertincta ?
-
 ?
additional information not: 3beta-hydroxysteroid, no electron acceptor: NADP+, K3Fe(CN)6, cytochrome c, 3-ketosteroids with a 11alpha- or 11beta-hydroxyl group are oxidized at slow rate, catalyzes aromatization of the A-ring of 19-nortestosterone and 19-norandrostenedione to produce estradiol and estrone, 19-hydroxytestosterone, 19-hydroxyandrosterone, 19-hydroxyandrostenedione and 19-oxotestosterone are converted to the respective phenolic steroids with cleavage of the C10 side-chain Gordonia rubripertincta ?
-
 ?
testololactone + acceptor D-homo-17a-oxoandrost-4-ene-3,17-dione Gordonia rubripertincta D-homo-17alpha-oxoandrosta-1,4-diene-3,17-one + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 60500, SDS-PAGE Gordonia rubripertincta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 activity increases linearly up to 45°C Gordonia rubripertincta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 20 min Gordonia rubripertincta
52
-
 20 min, 75% loss of activity Gordonia rubripertincta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 10 oxidase reaction Gordonia rubripertincta
10
-
 dehydrogenation, phenazine methosulfate Gordonia rubripertincta

pH Range

pH Minimum pH Maximum Comment Organism
7 10.5 pH 7: about 60% of activity maximum, pH 10.5: about 85% of activity maximum, phenazine methosulfate dehydrogenation Gordonia rubripertincta
8 11 pH 8: about 35% of activity maximum, pH 11: about 25% of activity maximum, oxidase reaction Gordonia rubripertincta

Cofactor

Cofactor Comment Organism Structure
FAD contains 1 mol of flavin adenine dinucleotide per mol of protein Gordonia rubripertincta