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Literature summary for 1.3.99.33 extracted from
- Urocanate reductase: identification of a novel anaerobic respiratory pathway in Shewanella oneidensis MR-1 (2012), Mol. Microbiol., 86, 1452-1463.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Shewanella oneidensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
Urocanate | Km less than 0.01 mM, at pH 7.0 and 25°C | Shewanella oneidensis |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Shewanella oneidensis | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, His-tagged enzyme, SDS-PAGE | Shewanella oneidensis |
| 60200 | - |
x * 60200, His-tagged enzyme, calculated from amino acid sequence | Shewanella oneidensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urocanate + reduced methyl viologen + H+ | Shewanella oneidensis | - |
dihydrourocanate + methyl viologen | - |
? | |
| urocanate + reduced methyl viologen + H+ | Shewanella oneidensis MR-1 / ATCC 700550 | - |
dihydrourocanate + methyl viologen | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Shewanella oneidensis | Q8CVD0 | - |
- |
| Shewanella oneidensis MR-1 / ATCC 700550 | Q8CVD0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, and gel filtration | Shewanella oneidensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is unable to reduce cinnamate and shows no activity with acrylic, cinnamic, crotonic, fumaric and orotic acids | Shewanella oneidensis | ? | - |
? | |
| additional information | the enzyme is unable to reduce cinnamate and shows no activity with acrylic, cinnamic, crotonic, fumaric and orotic acids | Shewanella oneidensis MR-1 / ATCC 700550 | ? | - |
? | |
| urocanate + reduced methyl viologen + H+ | - |
Shewanella oneidensis | dihydrourocanate + methyl viologen | - |
? | |
| urocanate + reduced methyl viologen + H+ | - |
Shewanella oneidensis MR-1 / ATCC 700550 | dihydrourocanate + methyl viologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, His-tagged enzyme, SDS-PAGE | Shewanella oneidensis |
| ? | x * 60200, His-tagged enzyme, calculated from amino acid sequence | Shewanella oneidensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| urdA | - |
Shewanella oneidensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 360 | - |
Urocanate | at pH 7.0 and 25°C | Shewanella oneidensis | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Shewanella oneidensis | |
| FMN | - |
Shewanella oneidensis | |
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Release 2023.1 (January 2023)
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