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Literature summary for 1.3.99.17 extracted from
- Replacement of active-site residues of quinoline 2-oxidoreductase involved in substrate recognition and specificity (2005), Curr. Microbiol., 50, 217-22.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into vector pUF1, expression in Escherichia coli DH5alpha pBL9 | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A259G | turnover rate 2.4fold reduced, no significant altered Km | Pseudomonas putida |
| A546G | turnover rate 199fold reduced, increased affinity for quinoline and quinoxaline | Pseudomonas putida |
| F375V | as stable as wild-type | Pseudomonas putida |
| V373A | turnover rate 40fold reduced | Pseudomonas putida |
| V373Y | as stable as wild-type | Pseudomonas putida |
| W331G | turnover rate 38fold reduced, suitable for hydroxylation of quinoxaline and C4-substituted quinolines | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.028 | - |
Quinoline | mutant A546G | Pseudomonas putida |  |
| 0.12 | - |
Quinoline | purified Qor protein | Pseudomonas putida | |
| 0.13 | - |
Quinoline | mutant A259G | Pseudomonas putida | |
| 0.33 | - |
Quinoline | mutant W331G | Pseudomonas putida | |
| 0.41 | - |
Quinoline | mutant V373A | Pseudomonas putida | |
| 0.68 | - |
Quinoxaline | mutantA546G | Pseudomonas putida | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida 86-1deltaqor | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to electrophoretic homogeneity | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.08 | - |
mutant A546G with quinoline as substrate | Pseudomonas putida |
| 0.36 | - |
mutant W331G with quinoline as substrate | Pseudomonas putida |
| 0.57 | - |
mutant V373A with quinoline as substrate | Pseudomonas putida |
| 16 | - |
mutant A259G with quinoline as substrate | Pseudomonas putida |
| 22 | - |
purified Qor protein with quinoline as substrate | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-methylquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 3-methylquinoline + acceptor + H2O | - |
Pseudomonas putida 86-1deltaqor | ? | - |
? | |
| 4-chloroquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 4-chloroquinoline + acceptor + H2O | - |
Pseudomonas putida 86-1deltaqor | ? | - |
? | |
| 4-hydroxyquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 4-hydroxyquinoline + acceptor + H2O | - |
Pseudomonas putida 86-1deltaqor | ? | - |
? | |
| 4-methylquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 4-methylquinoline + acceptor + H2O | - |
Pseudomonas putida 86-1deltaqor | ? | - |
? | |
| 5-carboxyquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 5-carboxyquinoline + acceptor + H2O | - |
Pseudomonas putida 86-1deltaqor | ? | - |
? | |
| 6-hydroxyquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 6-methylquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 7-hydroxyquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 8-hydroxyquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
| 8-methylquinoline + acceptor + H2O | - |
Pseudomonas putida | ? | - |
? | |
| quinoline + acceptor + H2O | - |
Pseudomonas putida | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
| quinoxaline + acceptor + H2O | - |
Pseudomonas putida | 2-oxoquinoxaline + reduced acceptor | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Qor | - |
Pseudomonas putida |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
Quinoline | mutant A546G | Pseudomonas putida | |
| 2.14 | - |
Quinoline | mutant V373A | Pseudomonas putida | |
| 2.2 | - |
Quinoxaline | mutantA546G | Pseudomonas putida | |
| 2.27 | - |
Quinoline | mutant W331G | Pseudomonas putida | |
| 35.7 | - |
Quinoline | mutant A259G | Pseudomonas putida | |
| 85.4 | - |
Quinoline | purified Qor protein | Pseudomonas putida | |
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