Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | 1 M, increases activity by about 10% | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | - |
Pseudomonas putida | |
methanol | 0.7 M, 50% inhibition | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.182 | - |
Quinoline | with 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride as cosubstrate | Pseudomonas putida | |
1.08 | - |
2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | with quinoline as cosubstrate | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | contains 8 atoms of iron and 8 atoms of acid-labile sulfide | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 20000 + x * 30000 + x * 85000, SDS-PAGE | Pseudomonas putida |
30000 | - |
x * 20000 + x * 30000 + x * 85000, SDS-PAGE | Pseudomonas putida |
85000 | - |
x * 20000 + x * 30000 + x * 85000, SDS-PAGE | Pseudomonas putida |
300000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida 86 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.2 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-chloroquinoline + p-iodonitrotetrazolium violet | - |
Pseudomonas putida | ? | - |
? | |
5-hydroxyquinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | ? | - |
? | |
5-hydroxyquinoline + acceptor | acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue | Pseudomonas putida | ? | - |
? | |
5-hydroxyquinoline + acceptor | 15% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
5-hydroxyquinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida 86 | ? | - |
? | |
5-hydroxyquinoline + acceptor | acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue | Pseudomonas putida 86 | ? | - |
? | |
6-hydroxyquinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | ? | - |
? | |
6-hydroxyquinoline + acceptor | acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue | Pseudomonas putida | ? | - |
? | |
6-hydroxyquinoline + acceptor | 19% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
7-hydroxyquinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | ? | - |
? | |
7-hydroxyquinoline + acceptor | acceptor: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue | Pseudomonas putida | ? | - |
? | |
8-chloroquinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | ? | - |
? | |
8-chloroquinoline + acceptor | acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue | Pseudomonas putida | ? | - |
? | |
8-hydroxyquinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | ? | - |
? | |
8-hydroxyquinoline + acceptor | acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,6-dichlorophenolindophenol, phenazine-methosulfate, methylene blue | Pseudomonas putida | ? | - |
? | |
quinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
quinoline + acceptor | acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride] | Pseudomonas putida | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
quinoline + acceptor | 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue | Pseudomonas putida | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
quinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida 86 | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
quinoline + acceptor | acceptors: 3,3'-(3,3'-dimethoxy-4,4'-bi-phenylene)-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride] | Pseudomonas putida 86 | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
quinoline + acceptor | 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue | Pseudomonas putida 86 | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20000 + x * 30000 + x * 85000, SDS-PAGE | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme contains 2 moleculs of FAD | Pseudomonas putida | |
molybdopterin | - |
Pseudomonas putida | |
additional information | NAD+ and NADP+ are ineffective as cofactors | Pseudomonas putida |