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Literature summary for 1.3.98.6 extracted from

  • Kühner, M.; Haufschildt, K.; Neumann, A.; Storbeck, S.; Streif, J.; Layer, G.
    The alternative route to heme in the methanogenic archaeon Methanosarcina barkeri (2014), Archaea, 2014, 327637 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.98.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Methanosarcina barkeri
expression in Escherichia coli Methanosarcina barkeri

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains 8.5 mol iron per mol enzyme Methanosarcina barkeri
Iron presence of 8.5 mol iron per mol AhbD and 5.1 mol sulfide per mol AhbD Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fe-coproporphyrin III + 2 S-adenosyl-L-methionine Methanosarcina barkeri
-
 protoheme + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine
-
 ?
Fe-coproporphyrin III + 2 S-adenosyl-L-methionine Methanosarcina barkeri DSM 804
-
 protoheme + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine
-
 ?
Fe-coproporphyrin III + S-adenosyl-L-methionine Methanosarcina barkeri
-
 protoheme + CO2 + 5'-deoxyadenosine + L-methionine
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
-
-
Methanosarcina barkeri Q46CH7
-
-
Methanosarcina barkeri DSM 804 Q46CH7
-
-

Purification (Commentary)

Purification (Comment) Organism
Blue Sepharose column chromatography and Mono Q column chromatography Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fe-coproporphyrin III + 2 S-adenosyl-L-methionine
-
 Methanosarcina barkeri protoheme + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine
-
 ?
Fe-coproporphyrin III + 2 S-adenosyl-L-methionine
-
 Methanosarcina barkeri DSM 804 protoheme + 2 CO2 + 2 5'-deoxyadenosine + 2 L-methionine
-
 ?
Fe-coproporphyrin III + S-adenosyl-L-methionine
-
 Methanosarcina barkeri protoheme + CO2 + 5'-deoxyadenosine + L-methionine
-
 ?

Synonyms

Synonyms Comment Organism
ahbD
-
 Methanosarcina barkeri
Mbar_A1458
-
 Methanosarcina barkeri

Cofactor

Cofactor Comment Organism Structure
4Fe-4S-center presence of two [4Fe-4S] clusters Methanosarcina barkeri
S-adenosyl-L-methionine
-
 Methanosarcina barkeri
[4Fe-4S]-center the enzyme contains two [4Fe-4S] clusters Methanosarcina barkeri