Cloned (Comment) | Organism |
---|---|
- |
Listeria monocytogenes serotype 1/2a |
Protein Variants | Comment | Organism |
---|---|---|
M149A | mutation alters the heme coordination, which becomes a 6-coordinate low spin species with the amide nitrogen atom of the Q187 residue bound to the heme iron. In the mutant, two CO conformers are present corresponding to open (A0) and closed (A1) conformations. Residue M149 is involved in the formation of a covalent bond with a vinyl substituent of heme b at excess of hydrogen peroxide | Listeria monocytogenes serotype 1/2a |
M149A/Q187A | binding of coproheme is significantly decreased. In the absence of the Q187, only the A0 conformer is found | Listeria monocytogenes serotype 1/2a |
Q187A | in the absence of the Q187, only the A0 conformer is found compared to two CO conformers are present corresponding to open (A0) and closed (A1) conformations in mutant M149A | Listeria monocytogenes serotype 1/2a |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the ferric form of the wild-type protein is a pentacoordinate quantum mechanically mixed-spin state | Listeria monocytogenes serotype 1/2a |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Listeria monocytogenes serotype 1/2a | Q8Y5F1 | - |
- |
Listeria monocytogenes serotype 1/2a ATCC BAA-679 | Q8Y5F1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fe-coproporphyrin III + 2 H2O2 | - |
Listeria monocytogenes serotype 1/2a | heme b + 2 CO2 + 4 H2O | - |
? | |
Fe-coproporphyrin III + 2 H2O2 | - |
Listeria monocytogenes serotype 1/2a ATCC BAA-679 | heme b + 2 CO2 + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChdC | - |
Listeria monocytogenes serotype 1/2a |
coproheme decarboxylase | - |
Listeria monocytogenes serotype 1/2a |
HemQ | - |
Listeria monocytogenes serotype 1/2a |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
Fe-coproporphyrin III | mutant M149A, pH 7, 25°C | Listeria monocytogenes serotype 1/2a | |
0.18 | - |
Fe-coproporphyrin III | wild-type, pH 7, 25°C | Listeria monocytogenes serotype 1/2a |