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Literature summary for 1.3.98.5 extracted from

  • Hofbauer, S.; Mlynek, G.; Milazzo, L.; Puehringer, D.; Maresch, D.; Schaffner, I.; Furtmueller, P.G.; Smulevich, G.; Djinovic-Carugo, K.; Obinger, C.
    Hydrogen peroxide-mediated conversion of coproheme to heme b by HemQ-lessons from the first crystal structure and kinetic studies (2016), FEBS J., 283, 4386-4401 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.98.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner (DE3) cells Listeria monocytogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme and enzyme in complex with Fe-coproporphyrin III, vapor diffusion method, using 0.1 M bicine pH 9.0, 10% (w/v) PEG6000 Listeria monocytogenes
HemQ crystallized as a pentamer in space group P1211, to 1.69 A resolution Listeria monocytogenes serotype 1/2a

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 H2O2 at pH 7.0 and 25°C Listeria monocytogenes
0.0371
-
 H2O2 pH 7.0, temperature not specified in the publication Listeria monocytogenes serotype 1/2a

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fe-coproporphyrin III + H2O2 Listeria monocytogenes
-
 harderoheme III + CO2 + H2O
-
 ?
Fe-coproporphyrin III + H2O2 Listeria monocytogenes overall reaction protoheme + CO2 + H2O
-
 ?
Fe-coproporphyrin III + H2O2 Listeria monocytogenes ATCC BAA-679
-
 harderoheme III + CO2 + H2O
-
 ?
Fe-coproporphyrin III + H2O2 Listeria monocytogenes ATCC BAA-679 overall reaction protoheme + CO2 + H2O
-
 ?
harderoheme III + H2O2 Listeria monocytogenes
-
 protoheme + CO2 + H2O
-
 ?
harderoheme III + H2O2 Listeria monocytogenes ATCC BAA-679
-
 protoheme + CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Listeria monocytogenes Q8Y5F1
-
-
Listeria monocytogenes ATCC BAA-679 Q8Y5F1
-
-
Listeria monocytogenes serotype 1/2a Q8Y5F1
-
-
Listeria monocytogenes serotype 1/2a ATCC BAA-679 Q8Y5F1
-
-

Purification (Commentary)

Purification (Comment) Organism
StrepTrap column chromatography Listeria monocytogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fe-coproporphyrin III + 2 H2O2
-
 Listeria monocytogenes serotype 1/2a protoheme + 2 CO2 + 4 H2O
-
 ?
Fe-coproporphyrin III + 2 H2O2
-
 Listeria monocytogenes serotype 1/2a ATCC BAA-679 protoheme + 2 CO2 + 4 H2O
-
 ?
Fe-coproporphyrin III + H2O2
-
 Listeria monocytogenes harderoheme III + CO2 + H2O
-
 ?
Fe-coproporphyrin III + H2O2
-
 Listeria monocytogenes ATCC BAA-679 harderoheme III + CO2 + H2O
-
 ?
Fe-coproporphyrin III + H2O2 overall reaction Listeria monocytogenes protoheme + CO2 + H2O
-
 ?
Fe-coproporphyrin III + H2O2 overall reaction Listeria monocytogenes ATCC BAA-679 protoheme + CO2 + H2O
-
 ?
harderoheme III + H2O2
-
 Listeria monocytogenes protoheme + CO2 + H2O
-
 ?
harderoheme III + H2O2
-
 Listeria monocytogenes ATCC BAA-679 protoheme + CO2 + H2O
-
 ?

Subunits

Subunits Comment Organism
homopentamer x-ray crystallography Listeria monocytogenes

Synonyms

Synonyms Comment Organism
HemQ
-
 Listeria monocytogenes
lmo2113
-
 Listeria monocytogenes serotype 1/2a

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0065
-
 H2O2 at pH 7.0 and 25°C Listeria monocytogenes
0.0065
-
 H2O2 pH 7.0, temperature not specified in the publication Listeria monocytogenes serotype 1/2a

Cofactor

Cofactor Comment Organism Structure
heme ferric five-coordinated coproheme iron of HemQ is weakly bound by a neutral proximal histidine H174 Listeria monocytogenes serotype 1/2a

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.18
-
 Fe-coproporphyrin III pH 7.0, temperature not specified in the publication Listeria monocytogenes serotype 1/2a
0.18
-
 H2O2 at pH 7.0 and 25°C Listeria monocytogenes