Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.98.1 extracted from

  • Cheleski, J.; Wiggers, H.J.; Citadini, A.P.; da Costa Filho, A.J.; Nonato, M.C.; Montanari, C.A.
    Kinetic mechanism and catalysis of Trypanosoma cruzi dihydroorotate dehydrogenase enzyme evaluated by isothermal titration calorimetry (2010), Anal. Biochem., 399, 13-22.
    View publication on PubMed

Application

Application Comment Organism
analysis protocol to measure enzyme kinetic parameters based on isothermal titration calorimetry. Presence of dimethyl sulfoxide at 10%, v/v and Triton X-100 at 0.5%, v/v seems to facilitate the substrate binding process with a small decrease in KM value Trypanosoma cruzi

Cloned(Commentary)

Cloned (Comment) Organism
-
Trypanosoma cruzi
expression of His-tagged enzyme Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information 10% v/v dimethyl sulfoxide and 0.5% v/vTriton X-100, which seem to facilitate the substrate binding process with a small decrease in Km, enzyme-substrate complex, pseudo-first-order kinetics, thermodynamics, overview Trypanosoma cruzi
0.00856
-
(S)-dihydroorotate assay based on isothermal titration calorimetry, pH 8.0, 25°C Trypanosoma cruzi
0.0086
-
L-dihydroorotate pH not specified in the publication, temperature not specified in the publication Trypanosoma cruzi
0.12
-
fumarate pH not specified in the publication, temperature not specified in the publication Trypanosoma cruzi
0.12
-
fumarate assay based on isothermal titration calorimetry, pH 8.0, 25°C Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Trypanosoma cruzi 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
-
Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-dihydroorotate + fumarate Trypanosoma cruzi DHODH catalyzes a coupled redox reaction in which dihydroorotate is oxidized to orotate and fumarate is reduced to succinate orotate + succinate
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
dimethyl sulfoxide presence of dimethyl sulfoxide at 10%, v/v and Triton X-100 at 0.5%, v/v during the assay seems to facilitate the substrate binding process with a small decrease in KM value Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme by nickel affinity chromatography Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + fumarate
-
Trypanosoma cruzi orotate + succinate
-
?
L-dihydroorotate + fumarate
-
Trypanosoma cruzi orotate + succinate
-
?
L-dihydroorotate + fumarate DHODH catalyzes a coupled redox reaction in which dihydroorotate is oxidized to orotate and fumarate is reduced to succinate Trypanosoma cruzi orotate + succinate
-
?

Subunits

Subunits Comment Organism
dimer
-
Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
DHODH
-
Trypanosoma cruzi
More a family 2 DHOD Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Trypanosoma cruzi