Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21-RP | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-orotate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
L-dihydroorotate | in 50mM Tris/HCl, 150 mM KCl, 0.1% (v/v) Triton X-100, pH 8, at 30°C | Saccharomyces cerevisiae | |
0.115 | - |
2,6-dichloroindophenol | in 50mM Tris/HCl, 150 mM KCl, 0.1% (v/v) Triton X-100, pH 8, at 30°C | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33900 | - |
2 * 33900, theoretical molecular mass | Saccharomyces cerevisiae |
67000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.06 | - |
using crotonate as electron acceptor | Saccharomyces cerevisiae |
0.94 | - |
using mesaconate as electron acceptor | Saccharomyces cerevisiae |
1.81 | - |
using O2 as electron acceptor | Saccharomyces cerevisiae |
5.1 | - |
using fumarate as electron acceptor | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-dihydroorotate + 2,6-dichlorophenolindophenol | - |
Saccharomyces cerevisiae | orotate + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-dihydroorotate + crotonate | - |
Saccharomyces cerevisiae | orotate + butanoate | - |
? | |
L-dihydroorotate + fumarate | fumarate is the most effective electron acceptor | Saccharomyces cerevisiae | orotate + succinate | - |
? | |
L-dihydroorotate + mesaconate | - |
Saccharomyces cerevisiae | orotate + 2-methylbutanedioate | - |
? | |
L-dihydroorotate + O2 | - |
Saccharomyces cerevisiae | orotate + H2O2 | - |
? | |
additional information | maleate, glutaconate, cis-aconitate, trans-aconitate, and cinnamate do not function as electron acceptor | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 33900, theoretical molecular mass | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
DHODH | - |
Saccharomyces cerevisiae |
DHODH-1A | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | contains 0.5 mol flavin per mol protein | Saccharomyces cerevisiae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0077 | - |
in 50mM Tris/HCl, 150 mM KCl, 0.1% (v/v) Triton X-100, pH 8, at 30°C | Saccharomyces cerevisiae | L-orotate |