Any feedback?
Literature summary for 1.3.98.1 extracted from
- Biosynthetic dihydroorotate dehydrogenase from Lactobacillus bulgaricus (1971), J. Bacteriol., 105, 1015-1027.
General Stability
| General Stability | Organism |
|---|---|
| 20% loss of activity after 1 h exposure to room light | Lactobacillus delbrueckii subsp. bulgaricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | visible light is inactivating | Lactobacillus delbrueckii subsp. bulgaricus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Lactobacillus delbrueckii subsp. bulgaricus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | enhances enzymatic activity up to a concentration of 0.0014 mM | Lactobacillus delbrueckii subsp. bulgaricus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
gel filtration, crude fraction | Lactobacillus delbrueckii subsp. bulgaricus |
| 57000 | - |
gel filtration | Lactobacillus delbrueckii subsp. bulgaricus |
| 57000 | - |
purified fraction | Lactobacillus delbrueckii subsp. bulgaricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-dihydroorotate + O2 | Lactobacillus delbrueckii subsp. bulgaricus | pathway of pyrimidine biosynthesis, constitutive biosynthetic enzyme | orotate + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus delbrueckii subsp. bulgaricus | - |
09, ATCC 13866 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion-exchange, gel filtration, ammonium sulfate precipitation | Lactobacillus delbrueckii subsp. bulgaricus |
| preparative gel electrophoresis | Lactobacillus delbrueckii subsp. bulgaricus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (S)-dihydroorotate + fumarate = orotate + succinate | light sensitivity seems to result in a conformational change and a partial loss of enzymatic activity | Lactobacillus delbrueckii subsp. bulgaricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 117.5 | - |
with ferricyanide as acceptor | Lactobacillus delbrueckii subsp. bulgaricus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, progressive enzyme decay | Lactobacillus delbrueckii subsp. bulgaricus |
| 4°C, purified enzyme, stable up to two years | Lactobacillus delbrueckii subsp. bulgaricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-dihydroorotate + ferricyanide | - |
Lactobacillus delbrueckii subsp. bulgaricus | orotate + ferrocyanide | - |
? | |
| (S)-dihydroorotate + O2 | redox dyes, 2,6-dichlorophenolindophenol, ferricyanide are most active electron acceptors, oxygen and cytochrome c are much slower | Lactobacillus delbrueckii subsp. bulgaricus | orotate + H2O2 | - |
? | |
| (S)-dihydroorotate + O2 | pathway of pyrimidine biosynthesis, constitutive biosynthetic enzyme | Lactobacillus delbrueckii subsp. bulgaricus | orotate + H2O2 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | 25 | assay at | Lactobacillus delbrueckii subsp. bulgaricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.7 | - |
S-dihydroorotate | with oxygen | Lactobacillus delbrueckii subsp. bulgaricus | |
| 157 | - |
ferricyanide | - |
Lactobacillus delbrueckii subsp. bulgaricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 7.8 | - |
Lactobacillus delbrueckii subsp. bulgaricus |
| 7.7 | - |
with ferricyanide as electron acceptor | Lactobacillus delbrueckii subsp. bulgaricus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | 1 mol per 55000 g of protein | Lactobacillus delbrueckii subsp. bulgaricus | |
| FMN | flavoprotein, enzyme contains 1 FMN as prosthetic group | Lactobacillus delbrueckii subsp. bulgaricus | |
| additional information | no FAD as prosthetic group | Lactobacillus delbrueckii subsp. bulgaricus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
