Inhibitors | Comment | Organism | Structure |
---|---|---|---|
palmitoyl-CoA | inhibition by micelle formation at concentrations up to 0.004 mM, reversed by fatty acid free bovine serum albumin | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
palmitoyl-CoA | - |
Bos taurus | |
0.001 | - |
palmitoyl-CoA | in the presence of fatty acid free bovine serum albumin | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
40-75% ammonium sulfate precipitation, G-200 electrophoresis | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Bos taurus | - |
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.076 | - |
purified enzyme, butyryl-CoA | Bos taurus |
0.19 | - |
purified enzyme, palmitoyl-CoA, 0.12 nmol/ml ETF | Bos taurus |
0.259 | - |
purified enzyme, palmitoyl-CoA, 0.24 nmol/ml ETF | Bos taurus |
0.272 | - |
purified enzyme, octanoyl-CoA | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyryl-CoA + electron-transfer flavoprotein | - |
Bos taurus | 2-butenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
octanoyl-CoA + electron-transfer flavoprotein | - |
Bos taurus | 2-octenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
palmitoyl-CoA + electron-transfer flavoprotein | - |
Bos taurus | 2-hexadecenoyl-CoA + reduced electron-transfer flavoprotein | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38000-45200, SDS-PAGE | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.67 | - |
palmitoyl-CoA | saturating ETF | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Bos taurus |