Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-hexadecenoyl-CoA | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
FAD | - |
Rattus norvegicus | |
0.0025 | - |
palmitoyl-CoA | - |
Rattus norvegicus | |
0.0054 | - |
stearoyl-CoA | - |
Rattus norvegicus | |
0.0065 | - |
oleoyl-CoA | - |
Rattus norvegicus | |
0.0074 | - |
tetradecanoyl-CoA | - |
Rattus norvegicus | |
0.0083 | - |
electron-transfer flavoprotein | - |
Rattus norvegicus | |
0.009 | - |
dodecanoyl-CoA | - |
Rattus norvegicus | |
0.0243 | - |
decanoyl-CoA | - |
Rattus norvegicus | |
0.123 | - |
octanoyl-CoA | - |
Rattus norvegicus | |
1 | - |
phenazine methosulfate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
40-80% ammonium sulfate precipitation, DEAE-Sephadex A-50, hydroxyapatite, matrex gel blue A, agarose-hexane-CoA, bio-gel A-0.5m | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.06 | - |
purified holoenzyme, arachidonoyl-CoA | Rattus norvegicus |
0.1 | - |
purified holoenzyme, erucoyl-CoA | Rattus norvegicus |
0.3 | - |
purified holoenzyme, docosanoyl-CoA | Rattus norvegicus |
0.38 | - |
purified holoenzyme, linolenoyl-CoA | Rattus norvegicus |
0.7 | - |
purified holoenzyme, octanoyl-CoA | Rattus norvegicus |
0.72 | - |
purified holoenzyme, linoleoyl-CoA | Rattus norvegicus |
0.9 | - |
purified holoenzyme, eicosanoyl-CoA | Rattus norvegicus |
1.13 | - |
purified holoenzyme, oleoyl-CoA | Rattus norvegicus |
1.5 | - |
purified holoenzyme, decanoyl-CoA | Rattus norvegicus |
1.51 | - |
purified holoenzyme, palmitoleoyl-CoA | Rattus norvegicus |
1.52 | - |
purified apoenzyme, palmitoyl-CoA | Rattus norvegicus |
1.6 | - |
purified holoenzyme, stearoyl-CoA | Rattus norvegicus |
1.8 | - |
purified holoenzyme, dodecanoyl-CoA | Rattus norvegicus |
1.96 | - |
purified holoenzyme, palmitoyl-CoA | Rattus norvegicus |
2.1 | - |
purified holoenzyme, tetradecanoyl-CoA | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E,5Z,8Z,11Z,14Z)-2,5,8,11,14-eicosapentaenoyl-CoA + reduced phenazine methosulfate | - |
? | |
decanoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-decenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
decanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E)-2-decenoyl-CoA + reduced phenazine methosulfate | - |
? | |
docosanoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-docosenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
docosanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-docosenoyl-CoA + reduced phenazine methosulfate | - |
? | |
dodecanoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-dodecenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
dodecanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E)-2-dodecenoyl-CoA + reduced phenazine methosulfate | - |
? | |
eicosanoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | trans-2-eicosenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
eicosanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | trans-2-eicosenoyl-CoA + reduced phenazine methosulfate | - |
? | |
erucoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E,13Z)-2,13-docosadienoyl-CoA + reduced phenazine methosulfate | - |
? | |
linolenoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E,6Z,9Z,12Z)2,6,9,12-octadecatetraenoyl-CoA + reduced phenazine methosulfate | - |
? | |
linoleoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E,9Z,12Z)-2,9,12-octadecatrienoyl-CoA + reduced phenazine methosulfate | - |
? | |
octanoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-octenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
octanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-octenoyl-CoA + reduced phenazine methosulfate | - |
? | |
oleoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E,9Z)-2,9-octadecadienoyl-CoA + reduced phenazine methosulfate | - |
? | |
palmitoleoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | (2E,9Z)-2,9-hexadecadienoyl-CoA + reduced phenazine methosulfate | - |
? | |
palmitoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-hexadecenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
palmitoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-hexadecenoyl-CoA + reduced phenazine methosulfate | - |
? | |
stearoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-octadecenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
stearoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-octadecenoyl-CoA + reduced phenazine methosulfate | - |
? | |
tetradecanoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-tetradecenoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
tetradecanoyl-CoA + phenazine methosulfate | - |
Rattus norvegicus | 2-tetradecenoyl-CoA + reduced phenazine methosulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38000-45200, SDS-PAGE | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.7 | stable for at least 10 min at 37°C | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rattus norvegicus |