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Literature summary for 1.3.8.8 extracted from

  • Ikeda, Y.; Okamura-Ikeda, K.; Tanaka, K.
    Purification and characterization of short-chain, medium-chain, and long-chain acyl-CoA dehydrogenases from rat liver mitochondria. Isolation of the holo- and apoenzymes and conversion of the apoenzyme to the holoenzyme (1985), J. Biol. Chem., 260, 1311-1325.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-hexadecenoyl-CoA
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
FAD
-
Rattus norvegicus
0.0025
-
palmitoyl-CoA
-
Rattus norvegicus
0.0054
-
stearoyl-CoA
-
Rattus norvegicus
0.0065
-
oleoyl-CoA
-
Rattus norvegicus
0.0074
-
tetradecanoyl-CoA
-
Rattus norvegicus
0.0083
-
electron-transfer flavoprotein
-
Rattus norvegicus
0.009
-
dodecanoyl-CoA
-
Rattus norvegicus
0.0243
-
decanoyl-CoA
-
Rattus norvegicus
0.123
-
octanoyl-CoA
-
Rattus norvegicus
1
-
phenazine methosulfate
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
40-80% ammonium sulfate precipitation, DEAE-Sephadex A-50, hydroxyapatite, matrex gel blue A, agarose-hexane-CoA, bio-gel A-0.5m Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
purified holoenzyme, arachidonoyl-CoA Rattus norvegicus
0.1
-
purified holoenzyme, erucoyl-CoA Rattus norvegicus
0.3
-
purified holoenzyme, docosanoyl-CoA Rattus norvegicus
0.38
-
purified holoenzyme, linolenoyl-CoA Rattus norvegicus
0.7
-
purified holoenzyme, octanoyl-CoA Rattus norvegicus
0.72
-
purified holoenzyme, linoleoyl-CoA Rattus norvegicus
0.9
-
purified holoenzyme, eicosanoyl-CoA Rattus norvegicus
1.13
-
purified holoenzyme, oleoyl-CoA Rattus norvegicus
1.5
-
purified holoenzyme, decanoyl-CoA Rattus norvegicus
1.51
-
purified holoenzyme, palmitoleoyl-CoA Rattus norvegicus
1.52
-
purified apoenzyme, palmitoyl-CoA Rattus norvegicus
1.6
-
purified holoenzyme, stearoyl-CoA Rattus norvegicus
1.8
-
purified holoenzyme, dodecanoyl-CoA Rattus norvegicus
1.96
-
purified holoenzyme, palmitoyl-CoA Rattus norvegicus
2.1
-
purified holoenzyme, tetradecanoyl-CoA Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E,5Z,8Z,11Z,14Z)-2,5,8,11,14-eicosapentaenoyl-CoA + reduced phenazine methosulfate
-
?
decanoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus 2-decenoyl-CoA + reduced electron-transfer flavoprotein
-
?
decanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E)-2-decenoyl-CoA + reduced phenazine methosulfate
-
?
docosanoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus 2-docosenoyl-CoA + reduced electron-transfer flavoprotein
-
?
docosanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-docosenoyl-CoA + reduced phenazine methosulfate
-
?
dodecanoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus 2-dodecenoyl-CoA + reduced electron-transfer flavoprotein
-
?
dodecanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E)-2-dodecenoyl-CoA + reduced phenazine methosulfate
-
?
eicosanoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus trans-2-eicosenoyl-CoA + reduced electron-transfer flavoprotein
-
?
eicosanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus trans-2-eicosenoyl-CoA + reduced phenazine methosulfate
-
?
erucoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E,13Z)-2,13-docosadienoyl-CoA + reduced phenazine methosulfate
-
?
linolenoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E,6Z,9Z,12Z)2,6,9,12-octadecatetraenoyl-CoA + reduced phenazine methosulfate
-
?
linoleoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E,9Z,12Z)-2,9,12-octadecatrienoyl-CoA + reduced phenazine methosulfate
-
?
octanoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus 2-octenoyl-CoA + reduced electron-transfer flavoprotein
-
?
octanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-octenoyl-CoA + reduced phenazine methosulfate
-
?
oleoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E,9Z)-2,9-octadecadienoyl-CoA + reduced phenazine methosulfate
-
?
palmitoleoyl-CoA + phenazine methosulfate
-
Rattus norvegicus (2E,9Z)-2,9-hexadecadienoyl-CoA + reduced phenazine methosulfate
-
?
palmitoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus 2-hexadecenoyl-CoA + reduced electron-transfer flavoprotein
-
?
palmitoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-hexadecenoyl-CoA + reduced phenazine methosulfate
-
?
stearoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus 2-octadecenoyl-CoA + reduced electron-transfer flavoprotein
-
?
stearoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-octadecenoyl-CoA + reduced phenazine methosulfate
-
?
tetradecanoyl-CoA + electron-transfer flavoprotein
-
Rattus norvegicus 2-tetradecenoyl-CoA + reduced electron-transfer flavoprotein
-
?
tetradecanoyl-CoA + phenazine methosulfate
-
Rattus norvegicus 2-tetradecenoyl-CoA + reduced phenazine methosulfate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 38000-45200, SDS-PAGE Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.7 stable for at least 10 min at 37°C Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Rattus norvegicus