Protein Variants | Comment | Organism |
---|---|---|
K304E | maximal velocity of the mutant protein is only one third of the wild-type value. 16% higher activity with the natural electron acceptor, electron transferring flavoprotein, than the wild-type enzyme. Decreased stability at temperatures above 42°C compared to wild-type enzyme | Rattus norvegicus |
Y42H | mutant enzyme has approximately the same maximal velocity as the wild-type enzyme, the KM-value for octanoyl-CoA is 1.6fold higher than the wild-type value. 7% higher activity with the natural electron acceptor, electron transferring flavoprotein, than the wild-type enzyme. Decreased stability at temperatures above 42°C compared to wild-type enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
octanoyl-CoA | wild-type enzyme | Rattus norvegicus | |
0.0059 | - |
octanoyl-CoA | mutant enzyme K304E | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | a Y42H mutation is frequently found in babies identified by newborn screening with MS/MS. Y42H is a temperature sensitive mutation, which is mild at low temperatures, but may have deleterious effects at increased temperatures | ? | - |
? | |
octanoyl-CoA + electron transferring flavoprotein | Rattus norvegicus | - |
2-octenoyl-CoA + reduced electron transferring flavoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | a Y42H mutation is frequently found in babies identified by newborn screening with MS/MS. Y42H is a temperature sensitive mutation, which is mild at low temperatures, but may have deleterious effects at increased temperatures | Rattus norvegicus | ? | - |
? | |
octanoyl-CoA + electron transferring flavoprotein | - |
Rattus norvegicus | 2-octenoyl-CoA + reduced electron transferring flavoprotein | - |
? | |
octanoyl-CoA + ferricenium | - |
Rattus norvegicus | 2-octenoyl-CoA + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MCAD | - |
Rattus norvegicus |
medium-chain acyl-CoA dehydrogenase | - |
Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
decreased stability of mutant enzymes Y42H and K304E above 42°C compared to wild-type enzyme | Rattus norvegicus |