Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-hexadecenoyl-CoA | competitive, long-chain acyl-CoA dehdrogenase | Rattus norvegicus | |
additional information | not inhibited by saturated and 2,3-unsaturated branched chain acyl-CoA compounds | Rattus norvegicus | |
octenoyl-CoA | competitive, medium-chain acyl-CoA dehydrogenase | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
octanoyl-CoA | - |
Rattus norvegicus | |
0.0045 | - |
electron transfer flavoprotein | - |
Rattus norvegicus | |
0.0054 | - |
decanoyl-CoA | - |
Rattus norvegicus | |
0.0057 | - |
dodecanoyl-CoA | - |
Rattus norvegicus | |
0.0094 | - |
hexanoyl-CoA | - |
Rattus norvegicus | |
0.0396 | - |
pentanoyl-CoA | - |
Rattus norvegicus | |
0.135 | - |
butanoyl-CoA | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
4 * 45000, SDS-PAGE | Rattus norvegicus |
180000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.96 | - |
long-chain acyl-CoA dehydrogenase | Rattus norvegicus |
10 | 10.2 | - |
Rattus norvegicus |
11.9 | - |
medium-chain acyl-CoA dehydrogenase | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + acceptor | electron acceptor: phenazine methosulfate | Rattus norvegicus | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
acyl-CoA + acceptor | intermediary electron acceptor: 2,6-dichloroindophenol | Rattus norvegicus | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
acyl-CoA + acceptor | final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue | Rattus norvegicus | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
butanoyl-CoA + acceptor | - |
Rattus norvegicus | crotonyl-CoA + reduced acceptor | - |
? | |
dodecanoyl-CoA + acceptor | - |
Rattus norvegicus | 2-dodecenoyl-CoA + reduced acceptor | - |
? | |
n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol | best substrate for medium-chain acyl-CoA dehydrogenase | Rattus norvegicus | 2-octenoyl-CoA + reduced acceptor | - |
? | |
n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol | no activity with tetradecanoyl-CoA and hexadecanoyl-CoA | Rattus norvegicus | 2-octenoyl-CoA + reduced acceptor | - |
? | |
n-tetradecanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol | best substrate for long-chain acyl-CoA dehydrogenase | Rattus norvegicus | 2-tetradecenoyl-CoA + reduced acceptor | - |
? | |
pentanoyl-CoA + acceptor | - |
Rattus norvegicus | 2-pentenoyl-CoA + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 45000, SDS-PAGE | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
tetradecanoyl-CoA | long-chain acyl-CoA dehydrogenase | Rattus norvegicus | |
31.2 | - |
hexanoyl-CoA | medium-chain acyl-CoA dehydrogenase | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.7 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol per mol of subunit | Rattus norvegicus |