Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(methylenecyclopropyl)acetyl-CoA | 0.01 mM, 95% inhibition | Homo sapiens | |
(R)-(-)-(methylenecyclopropyl)acetyl-CoA | - |
Homo sapiens | |
iodoacetamide | 2 mM, 73% inhibition | Homo sapiens | |
N-ethylmaleimide | 2 mM, 43% inhibition | Homo sapiens | |
p-chloromercuribenzoate | 0.1 mM, 17% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
electron transfer flavoprotein | - |
Homo sapiens | |
0.00714 | - |
butyryl-CoA | - |
Homo sapiens | |
0.008 | - |
hexanoyl-CoA | - |
Homo sapiens | |
0.0091 | - |
decanoyl-CoA | - |
Homo sapiens | |
0.01 | - |
tetradecenoyl-CoA | - |
Homo sapiens | |
0.0216 | - |
hexanoyl-CoA | - |
Homo sapiens | |
0.023 | - |
palmitoyl-CoA | - |
Homo sapiens | |
0.0563 | - |
valeryl-CoA | - |
Homo sapiens | |
1.67 | - |
phenazine methosulfate | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
4 * 44000, SDS-PAGE | Homo sapiens |
178000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.75 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + acceptor | electron acceptor: phenazine methosulfate | Homo sapiens | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
acyl-CoA + acceptor | chain-length specificity depending on electron acceptor: with phenazine methosulfate hexanoyl-CoA, with electron transfer flavoprotein, octanoyl-CoA | Homo sapiens | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
acyl-CoA + acceptor | intermediary electron acceptor: 2,6-dichloroindophenol | Homo sapiens | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
acyl-CoA + acceptor | final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue | Homo sapiens | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
butanoyl-CoA + acceptor | - |
Homo sapiens | crotonyl-CoA + reduced acceptor | - |
? | |
decanoyl-CoA + acceptor | acceptors: phenazine methosulfate or electron transferring flavoprotein | Homo sapiens | 2-decenoyl-CoA + reduced acceptor | - |
? | |
dodecanoyl-CoA + acceptor | acceptors: phenazine methosulfate or electron transferring flavoprotein | Homo sapiens | 2-dodecenoyl-CoA + reduced acceptor | - |
? | |
hexadecanoyl-CoA + acceptor | acceptors: phenazine methosulfate or electron transferring flavoprotein, very low activity | Homo sapiens | 2-hexadecenoyl-CoA + reduced acceptor | - |
? | |
hexanoyl-CoA + acceptor | acceptors: phenazine methosulfate or electron transferring flavoprotein, best substrate with phenazine methosulfate | Homo sapiens | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
n-octanoyl-CoA + phenazine methosulfate + 2,6-dichloroindophenol | best substrate with electron transferring flavoprotein | Homo sapiens | 2-octenoyl-CoA + reduced acceptor | - |
? | |
octadecanoyl-CoA + acceptor | acceptors: phenazine methosulfate or electron transferring flavoprotein, extremely low activity | Homo sapiens | 2-octadecenoyl-CoA + reduced acceptor | - |
? | |
pentanoyl-CoA + acceptor | acceptors: phenazine methosulfate or electron transferring flavoprotein | Homo sapiens | 2-pentenoyl-CoA + reduced acceptor | - |
? | |
tetradecanoyl-CoA + acceptor | acceptors: phenazine methosulfate or electron transferring flavoprotein, low activity | Homo sapiens | 2-tetradecenoyl-CoA + reduced acceptor | - |
? | |
tetradecenoyl-CoA + reduced electron transfer flavoprotein | - |
Homo sapiens | tetradecanoyl-CoA + electron transfer flavoprotein | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 44000, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens |