Protein Variants | Comment | Organism |
---|---|---|
E370A | site-directed mutagenesis, inactive mutant | Rattus norvegicus |
E370Q | site-directed mutagenesis, the mutant shows highy reduced activity compared to the wild-type enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-pentynoyl-CoA | inactivates GCD | Rattus norvegicus | |
oct-2-yn-4-enoyl-CoA | irreversible and most likely involved covalent modification of the apoenzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics with medium chain acyl-CoA substrates, overview | Rattus norvegicus | |
0.035 | - |
glutaryl-CoA | mutant E370Q, pH and temperature not specified in the publication | Rattus norvegicus | |
3.8 | - |
glutaryl-CoA | wild-type enzyme, pH and temperature not specified in the publication | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-CoA + FAD | Rattus norvegicus | GCD catalyzes the oxidative decarboxylation of the gamma-carboxylate of the substrate, glutaryl-CoA, to yield crotonyl-CoA and CO2. | (E)-but-2-enoyl-CoA + CO2 + FADH2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-CoA + FAD | - |
Rattus norvegicus | (E)-but-2-enoyl-CoA + CO2 + FADH2 | - |
? | |
glutaryl-CoA + FAD | GCD catalyzes the oxidative decarboxylation of the gamma-carboxylate of the substrate, glutaryl-CoA, to yield crotonyl-CoA and CO2. | Rattus norvegicus | (E)-but-2-enoyl-CoA + CO2 + FADH2 | - |
? | |
additional information | the enzyme is also active with butyryl-CoA, hexanoyl-CoA, octanoyl-CoA, and decanoyl-CoA, but is inactive with decanoyl-CoA and dodecanoyl-CoA, substrate specificity, overview | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GCD | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
glutaryl-CoA | wild-type enzyme, pH and temperature not specified in the publication | Rattus norvegicus | |
0.012 | - |
glutaryl-CoA | mutant E370Q, pH and temperature not specified in the publication | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the acyl-CoA dehydrogenase (ACD) family of flavoproteins | Rattus norvegicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
glutaryl-CoA | mutant E370Q, pH and temperature not specified in the publication | Rattus norvegicus | |
950 | - |
glutaryl-CoA | wild-type enzyme, pH and temperature not specified in the publication | Rattus norvegicus |