Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain XL1Blue | Solanum tuberosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
2-methylbutanoyl-CoA | recombinant enzyme, 32°C | Solanum tuberosum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 50000, about, SDS-PAGE | Solanum tuberosum |
188000 | - |
recombinant enzyme, gel filtration | Solanum tuberosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli to over 90% purity | Solanum tuberosum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-methylbutanoyl-CoA + electron-transfer flavoprotein = (E)-2-methylbut-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ | the isovaleryl-CoA dehydrogenase 1, i.e. IVD1, EC 1.3.99.10, from Solanum tuberosum shows maximal activity with 2-methylbutyryl-CoA, the binding pockets of 2-methylbutyryl-CoA and isovaleryl-CoA dehydrogenase are nearly identical, the enzyme therefore shall also be classified to EC 1.3.99.12, active site structure, residues Met361, Ala365, Val88, Val92, and Val96 are involved in catalysis | Solanum tuberosum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
sprout | - |
Solanum tuberosum | - |
tuber | - |
Solanum tuberosum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Solanum tuberosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylbutanoyl-CoA + electron transfer flavoprotein | - |
Solanum tuberosum | 2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein | - |
? | |
butyryl-CoA + electron-transfer flavoprotein | - |
Solanum tuberosum | but-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ | - |
? | |
hexanoyl-CoA + electron-transfer flavoprotein | - |
Solanum tuberosum | hex-2-enoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
additional information | no activity with isovaleryl-CoA | Solanum tuberosum | ? | - |
? | |
octanoyl-CoA + electron-transfer flavoprotein | - |
Solanum tuberosum | oct-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ | - |
? | |
valeryl-CoA + electron-transfer flavoprotein | - |
Solanum tuberosum | pent-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50000, about, SDS-PAGE | Solanum tuberosum |
Synonyms | Comment | Organism |
---|---|---|
2-methylbutyryl-CoA dehydrogenase | - |
Solanum tuberosum |
2MBCD | - |
Solanum tuberosum |
SBCAD | - |
Solanum tuberosum |
short/branched chain acyl-CoA dehydrogenase | - |
Solanum tuberosum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.77 | - |
2-methylbutanoyl-CoA | per enzyme tetramer, recombinant enzyme, 32°C | Solanum tuberosum |