Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(E)-2-methyl-2-butenoyl-CoA | - |
Ascaris suum | |
acetoacetyl-CoA | - |
Ascaris suum | |
Ag2+ | - |
Ascaris suum | |
crotonyl-CoA | - |
Ascaris suum | |
decanoyl-CoA | - |
Ascaris suum | |
hexanoyl-CoA | - |
Ascaris suum | |
Hg2+ | - |
Ascaris suum | |
NEM | little effect | Ascaris suum | |
p-hydroxymercuribenzoate | little effect | Ascaris suum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
2-methylbutanoyl-CoA | - |
Ascaris suum | |
0.021 | - |
2-methylpentanoyl-CoA | - |
Ascaris suum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Ascaris suum | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42500 | - |
4 * 42500, SDS-PAGE | Ascaris suum |
170000 | - |
gel filtration | Ascaris suum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ascaris suum | the electron-transport chain of the organism and the enzyme 2-methyl-branched-chain-enoyl-CoA reductase are responsible for the NADH-dependent reduction of the branched-chain enoyl-CoAs observed in mitochondria | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ascaris suum | O16843 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Ascaris suum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.58 | - |
2-methylpentanoyl-CoA | Ascaris suum |
1.62 | - |
2-methylbutanoyl-CoA | Ascaris suum |
Storage Stability | Organism |
---|---|
-20°C, 50 mM potassium phosphate buffer, pH 7.4, 10% glycerol, stable for at least 30 days | Ascaris suum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylbutanoyl-CoA + electron-transfer flavoprotein | - |
Ascaris suum | (E)-2-methylbut-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ | - |
? | |
2-methylpentanoyl-CoA + electron-transfer flavoprotein | 98% of the activity with 2-methylbutanoyl-CoA | Ascaris suum | 2-methyl-2-pentenoyl-CoA + reduced electron-transfer flavoprotein + H+ | - |
? | |
butyryl-CoA + electron-transfer flavoprotein | 13.6% of the activity with 2-methylbutanoyl-CoA | Ascaris suum | ? + reduced electron-transfer flavoprotein + H+ | - |
? | |
additional information | no reaction with: propionyl-CoA, isobutanoyl-CoA, isopentanoyl-CoA, palmitoyl-CoA | Ascaris suum | ? | - |
? | |
additional information | the electron-transport chain of the organism and the enzyme 2-methyl-branched-chain-enoyl-CoA reductase are responsible for the NADH-dependent reduction of the branched-chain enoyl-CoAs observed in mitochondria | Ascaris suum | ? | - |
? | |
octanoyl-CoA + electron-transfer flavoprotein | 12.8% of the activity with 2-methylbutanoyl-CoA | Ascaris suum | ? + reduced electron-transfer flavoprotein + H+ | - |
? | |
pentanoyl-CoA + electron-transfer flavoprotein | 14.4% of the activity with 2-methylbutanoyl-CoA | Ascaris suum | ? + reduced electron-transfer flavoprotein + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42500, SDS-PAGE | Ascaris suum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Ascaris suum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 0.9 mol of FAD per mol of subunit | Ascaris suum |