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Literature summary for 1.3.8.4 extracted from

  • Urano, K.; Daimon, T.; Banno, Y.; Mita, K.; Terada, T.; Shimizu, K.; Katsuma, S.; Shimada, T.
    Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori (2010), FEBS J., 277, 4452-4463.
    View publication on PubMed
Search for more literature for 1.3.8.4

Cloned(Commentary)

Cloned (Comment) Organism
IVD gene, DNA and amino acid sequence deteremination and analysis, alignment of BmIVD with all 11 human acyl-CoA dehydrogenase family members, overview. Expression of wild-type BmIVD and sku-type BmIVD in Spodoptera frugiperda Sf9 cells via transfection with baculovrius Bombyx mori

Protein Variants

Protein Variants Comment Organism
G376V the mutation has negative effects on FAD-binding or on monomer-monomer interactions of the skunk mutant enzyme, like in strain a85, the mutant shows a odorous phenotype with prepupal lethality Bombyx mori

Organism

Organism UniProt Comment Textmining
Bombyx mori B5UB85
-
-
Bombyx mori c108T B5UB85
-
-
Bombyx mori p50T B5UB85
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information activity measurement using an acyl-CoA substrate and 2,6-dichloroindophenol as an electron acceptor, with phenazinemethosulfate as an intermediate electron carrier Bombyx mori ?
-
 ?
additional information activity measurement using an acyl-CoA substrate and 2,6-dichloroindophenol as an electron acceptor, with phenazinemethosulfate as an intermediate electron carrier Bombyx mori p50T ?
-
 ?
additional information activity measurement using an acyl-CoA substrate and 2,6-dichloroindophenol as an electron acceptor, with phenazinemethosulfate as an intermediate electron carrier Bombyx mori c108T ?
-
 ?

Synonyms

Synonyms Comment Organism
IVD
-
 Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Bombyx mori

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Bombyx mori

General Information

General Information Comment Organism
metabolism IVD acts in the third step of leucine degradation Bombyx mori
additional information a G376V molecular defect of isovaleryl-CoA dehydrogenase, IVD, causes IVD deficiency which is responsible for the isovaleric acid-emanating skunk mutant odorous phenotype with prepupal lethality of the silkworm, molecular modelling, overview. The sku larvae begins emanating isovaleric acid odour from the first day after hatching, but does not show any signs of developmental abnormality until the onset of spinning. The mutants start spinning after the normal duration of the final instar, 6-8 days, but stop after a short time and develop a very thin cocoon. They eventually die without becoming pupae in about a week after spinning Bombyx mori