Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Solanum tuberosum |
expression of wild-type and mutants in Escherichia coli strain JM105 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L370M/G374A | site-directed mutagenesis, substrate specificity similar to the wild-type enzyme, reduced activity | Homo sapiens |
L95V/A99V/L103V | site-directed mutagenesis, inactive mutant | Homo sapiens |
L95V/A99V/L103V/L370M/G374A | site-directed mutagenesis, substitutions in the human enzyme mimick the potato isovaleryl-CoA dehydrogenase isozyme 1, which shows major 2-methylbutyryl-CoA dehydrogenase activity and rather belongs to EC 1.3.99.12, the mutant enzymes shows modified substrate specificty and also exhibits highest activity with 2-methylbutanoyl-CoA, molecular modeling of the active site | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
isovaleryl-CoA | per tetramer, recombinant enzyme, 32°C | Solanum tuberosum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 50000, about, recombinant enzyme, SDS-PAGE | Solanum tuberosum |
146000 | - |
recombinant enzyme, gel filtration | Homo sapiens |
146000 | - |
recombinant enzyme, gel filtration | Solanum tuberosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isovaleryl-CoA + electron transfer protein | Homo sapiens | - |
3-methylcrotonoyl-CoA + reduced electron transfer protein | - |
? | |
isovaleryl-CoA + electron transfer protein | Solanum tuberosum | - |
3-methylcrotonyl-CoA + reduced electron transfer protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Solanum tuberosum | Q9FS87 | isozyme IVD2 | - |
Purification (Comment) | Organism |
---|---|
partially from tuber, recombinant from Escherichia coli to over 90% purity | Solanum tuberosum |
recombinant from Escherichia coli to over 90% purity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
sprout | - |
Solanum tuberosum | - |
tuber | - |
Solanum tuberosum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0003 | - |
recombinant mutant L95V/A99V/L103V/L370M/G374A, substrate 2-methylbutanoyl-CoA | Homo sapiens |
0.0011 | - |
recombinant mutant L370M/G374A, substrate 2-methylbutanoyl-CoA | Homo sapiens |
0.0043 | - |
recombinant wild-type enzyme, substrate 2-methylbutanoyl-CoA | Homo sapiens |
0.0114 | - |
recombinant mutant L370M/G374A, substrate butyryl-CoA | Homo sapiens |
0.0152 | - |
recombinant wild-type enzyme, substrate butyryl-CoA | Homo sapiens |
0.0239 | - |
recombinant mutant L370M/G374A, substrate valeryl-CoA | Homo sapiens |
0.0364 | - |
recombinant wild-type enzyme, substrate valeryl-CoA | Homo sapiens |
0.0371 | - |
recombinant mutant L370M/G374A, substrate isovaleryl-CoA | Homo sapiens |
0.0754 | - |
recombinant wild-type enzyme, substrate isovaleryl-CoA | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylbutanoyl-CoA + acceptor | low activity with the wild-type and mutant L95V/A99V/L103V/L370M/G374A | Homo sapiens | 2-methylbut-2-enoyl-CoA + reduced acceptor | - |
? | |
butyryl-CoA + acceptor | - |
Homo sapiens | but-2-enoyl-CoA + reduced acceptor | - |
? | |
butyryl-CoA + acceptor | low activity | Solanum tuberosum | but-2-enoyl-CoA + reduced acceptor | - |
? | |
hexanoyl-CoA + acceptor | low activity | Solanum tuberosum | hex-2-enoyl-CoA + reduced acceptor | - |
? | |
isovaleryl-CoA + electron transfer protein | - |
Homo sapiens | 3-methylcrotonoyl-CoA + reduced electron transfer protein | - |
? | |
isovaleryl-CoA + electron transfer protein | - |
Solanum tuberosum | 3-methylcrotonyl-CoA + reduced electron transfer protein | - |
? | |
isovaleryl-CoA + electron transfer protein | best substrate | Solanum tuberosum | 3-methylcrotonyl-CoA + reduced electron transfer protein | - |
? | |
additional information | substrate specificity of wild-type and mutant enzymes | Homo sapiens | ? | - |
? | |
octanoyl-CoA + acceptor | very low activity | Solanum tuberosum | oct-2-enoyl-CoA + reduced acceptor | - |
? | |
valeryl-CoA + acceptor | - |
Homo sapiens | pent-2-enoyl-CoA + reduced acceptor | - |
? | |
valeryl-CoA + acceptor | low activity | Solanum tuberosum | pent-2-enoyl-CoA + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 50000, about, recombinant enzyme, SDS-PAGE | Solanum tuberosum |
Synonyms | Comment | Organism |
---|---|---|
IVD | - |
Homo sapiens |
IVD | - |
Solanum tuberosum |
SBCAD | - |
Homo sapiens |
short/branched chain acyl-CoA dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
assay at | Solanum tuberosum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.98 | - |
isovaleryl-CoA | per tetramer, recombinant enzyme, 32°C | Solanum tuberosum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
electron transfer protein | - |
Homo sapiens | |
electron transfer protein | - |
Solanum tuberosum |