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Literature summary for 1.3.8.4 extracted from

  • Goetzman, E.S.; Mohsen, A.W.; Prasad, K.; Vockley, J.
    Convergent evolution of a 2-methylbutyryl-CoA dehydrogenase from isovaleryl-CoA dehydrogenase in Solanum tuberosum (2005), J. Biol. Chem., 280, 4873-4879.
    View publication on PubMed
Search for more literature for 1.3.8.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Solanum tuberosum
expression of wild-type and mutants in Escherichia coli strain JM105 Homo sapiens

Protein Variants

Protein Variants Comment Organism
L370M/G374A site-directed mutagenesis, substrate specificity similar to the wild-type enzyme, reduced activity Homo sapiens
L95V/A99V/L103V site-directed mutagenesis, inactive mutant Homo sapiens
L95V/A99V/L103V/L370M/G374A site-directed mutagenesis, substitutions in the human enzyme mimick the potato isovaleryl-CoA dehydrogenase isozyme 1, which shows major 2-methylbutyryl-CoA dehydrogenase activity and rather belongs to EC 1.3.99.12, the mutant enzymes shows modified substrate specificty and also exhibits highest activity with 2-methylbutanoyl-CoA, molecular modeling of the active site Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 isovaleryl-CoA per tetramer, recombinant enzyme, 32°C Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 4 * 50000, about, recombinant enzyme, SDS-PAGE Solanum tuberosum
146000
-
 recombinant enzyme, gel filtration Homo sapiens
146000
-
 recombinant enzyme, gel filtration Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isovaleryl-CoA + electron transfer protein Homo sapiens
-
 3-methylcrotonoyl-CoA + reduced electron transfer protein
-
 ?
isovaleryl-CoA + electron transfer protein Solanum tuberosum
-
 3-methylcrotonyl-CoA + reduced electron transfer protein
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Solanum tuberosum Q9FS87 isozyme IVD2
-

Purification (Commentary)

Purification (Comment) Organism
partially from tuber, recombinant from Escherichia coli to over 90% purity Solanum tuberosum
recombinant from Escherichia coli to over 90% purity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
sprout
-
 Solanum tuberosum
-
tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0003
-
 recombinant mutant L95V/A99V/L103V/L370M/G374A, substrate 2-methylbutanoyl-CoA Homo sapiens
0.0011
-
 recombinant mutant L370M/G374A, substrate 2-methylbutanoyl-CoA Homo sapiens
0.0043
-
 recombinant wild-type enzyme, substrate 2-methylbutanoyl-CoA Homo sapiens
0.0114
-
 recombinant mutant L370M/G374A, substrate butyryl-CoA Homo sapiens
0.0152
-
 recombinant wild-type enzyme, substrate butyryl-CoA Homo sapiens
0.0239
-
 recombinant mutant L370M/G374A, substrate valeryl-CoA Homo sapiens
0.0364
-
 recombinant wild-type enzyme, substrate valeryl-CoA Homo sapiens
0.0371
-
 recombinant mutant L370M/G374A, substrate isovaleryl-CoA Homo sapiens
0.0754
-
 recombinant wild-type enzyme, substrate isovaleryl-CoA Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylbutanoyl-CoA + acceptor low activity with the wild-type and mutant L95V/A99V/L103V/L370M/G374A Homo sapiens 2-methylbut-2-enoyl-CoA + reduced acceptor
-
 ?
butyryl-CoA + acceptor
-
 Homo sapiens but-2-enoyl-CoA + reduced acceptor
-
 ?
butyryl-CoA + acceptor low activity Solanum tuberosum but-2-enoyl-CoA + reduced acceptor
-
 ?
hexanoyl-CoA + acceptor low activity Solanum tuberosum hex-2-enoyl-CoA + reduced acceptor
-
 ?
isovaleryl-CoA + electron transfer protein
-
 Homo sapiens 3-methylcrotonoyl-CoA + reduced electron transfer protein
-
 ?
isovaleryl-CoA + electron transfer protein
-
 Solanum tuberosum 3-methylcrotonyl-CoA + reduced electron transfer protein
-
 ?
isovaleryl-CoA + electron transfer protein best substrate Solanum tuberosum 3-methylcrotonyl-CoA + reduced electron transfer protein
-
 ?
additional information substrate specificity of wild-type and mutant enzymes Homo sapiens ?
-
 ?
octanoyl-CoA + acceptor very low activity Solanum tuberosum oct-2-enoyl-CoA + reduced acceptor
-
 ?
valeryl-CoA + acceptor
-
 Homo sapiens pent-2-enoyl-CoA + reduced acceptor
-
 ?
valeryl-CoA + acceptor low activity Solanum tuberosum pent-2-enoyl-CoA + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, about, recombinant enzyme, SDS-PAGE Solanum tuberosum

Synonyms

Synonyms Comment Organism
IVD
-
 Homo sapiens
IVD
-
 Solanum tuberosum
SBCAD
-
 Homo sapiens
short/branched chain acyl-CoA dehydrogenase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
 assay at Solanum tuberosum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.98
-
 isovaleryl-CoA per tetramer, recombinant enzyme, 32°C Solanum tuberosum

Cofactor

Cofactor Comment Organism Structure
electron transfer protein
-
 Homo sapiens
electron transfer protein
-
 Solanum tuberosum