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Literature summary for 1.3.8.4 extracted from

  • Ikeda, Y.; Tanaka, K.
    Purification and characterization of isovaleryl coenzyme A dehydrogenase from rat liver mitochondria (1983), J. Biol. Chem., 258, 1077-1085.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(methylenecyclopropyl)acetyl-CoA completely inhibits at 0.01 mM; strongly inhibits at 0.01 mM Rattus norvegicus
3-methylcrotonyl-CoA
-
Rattus norvegicus
Ag+ completely inhibits at 0.01 mM Rattus norvegicus
Cu2+ completely inhibits at 0.01 mM Rattus norvegicus
Hg2+ completely inhibits at 0.1 mM Rattus norvegicus
Methylmercury iodide
-
Rattus norvegicus
N-ethylmaleimide
-
Rattus norvegicus
p-hydroxymercuribenzoate
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0167 0.4 n-valeryl-CoA
-
Rattus norvegicus
0.033
-
isovaleryl-CoA with phenazine methosulfate as an artificial electron acceptor Rattus norvegicus
0.4
-
n-valeryl-CoA
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
4 * 43000, SDS-PAGE Rattus norvegicus
175000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isovaleryl-CoA + acceptor Rattus norvegicus electron acceptor: electron-transfer flavoprotein serves as a natural electron acceptor for the enzyme with isovaleryl-CoA as substrate 3-methylcrotonyl-CoA + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using sonication, ammonium sulfate treatment and column chromatography on DEAE-Sephadex A-50, hydroxylapatite, Matrex Gel Blue A and BioGel A-0.5 m Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.086
-
-
Rattus norvegicus
0.832
-
-
Rattus norvegicus
2.68
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, 1 month Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butyryl-CoA + acceptor 3.3% relative activity to isovaleryl-CoA Rattus norvegicus but-2-enoyl-CoA + reduced acceptor
-
?
isovaleryl-CoA + acceptor isovaleryl-CoA is the preferred substrate Rattus norvegicus 3-methylcrotonyl-CoA + reduced acceptor
-
?
isovaleryl-CoA + acceptor acceptors: electron-transfer flavoprotein, phenazine methosulfate Rattus norvegicus 3-methylcrotonyl-CoA + reduced acceptor
-
?
isovaleryl-CoA + acceptor electron acceptor: electron-transfer flavoprotein serves as a natural electron acceptor for the enzyme with isovaleryl-CoA as substrate Rattus norvegicus 3-methylcrotonyl-CoA + reduced acceptor
-
?
n-valeryl-CoA + acceptor 32% relative specific activity to isovaleryl-CoA Rattus norvegicus pent-2-enoyl-CoA + reduced acceptor
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 43000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
isovaleryl-CoA + phenazine methosulfate Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme contains FAD as a prosthetic group, 0.6 mol FAD per mol of subunit Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02
-
3-methylcrotonyl-CoA
-
Rattus norvegicus