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Literature summary for 1.3.8.10 extracted from

  • Kung, J.W.; Meier, A.K.; Mergelsberg, M.; Boll, M.
    Enzymes involved in a novel anaerobic cyclohexane carboxylic acid degradation pathway (2014), J. Bacteriol., 196, 3667-3674 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
cyclohex-1-ene-1-carbonyl-CoA pH 6.8, 30°C Geobacter metallireducens
0.085
-
cyclohex-1,5-diene-1-carbonyl-CoA pH 6.8, 30°C Geobacter metallireducens

Organism

Organism UniProt Comment Textmining
Geobacter metallireducens Q39QF5
-
-
Geobacter metallireducens DSM 7210 Q39QF5
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
substrate cyclohex-1,5-diene-1-carbonyl-CoA, pH 6.8, 30°C Geobacter metallireducens
2.3
-
substrate cyclohex-1-ene-1-carbonyl-CoA, pH 6.8, 30°C Geobacter metallireducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclohex-1,5-diene-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
Geobacter metallireducens benzoyl-CoA + ferrocenium hexafluorophosphate
-
?
cyclohex-1,5-diene-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
Geobacter metallireducens DSM 7210 benzoyl-CoA + ferrocenium hexafluorophosphate
-
?
cyclohex-1-ene-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
Geobacter metallireducens cyclohex-1,5-diene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
?
cyclohex-1-ene-1-carbonyl-CoA + ferricenium hexafluorophosphate
-
Geobacter metallireducens DSM 7210 cyclohex-1,5-diene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
-
?
additional information no substrates: cyclohexanoyl-CoA, cyclohex-2-ene-1-carboxyl-CoA, cyclohex-3-ene-1-carboxyl-CoA, butanoyl-CoA, nor crotonyl-CoA Geobacter metallireducens ?
-
?
additional information no substrates: cyclohexanoyl-CoA, cyclohex-2-ene-1-carboxyl-CoA, cyclohex-3-ene-1-carboxyl-CoA, butanoyl-CoA, nor crotonyl-CoA Geobacter metallireducens DSM 7210 ?
-
?

Subunits

Subunits Comment Organism
? x * 40900,SDS-PAGE, recombinant protein with His-tag Geobacter metallireducens

Synonyms

Synonyms Comment Organism
Gmet_3306
-
Geobacter metallireducens

General Information

General Information Comment Organism
physiological function Geobacter metallireducens grows exponentially with cyclohexane carboxylic acid as the sole carbon source and nitrate as a terminal electron acceptor. Cyclohexane carboxylic acid degradation involves its activation by a succinyl-CoA-dependent CoA transferase followed by the oxidation to cyclohexadienoyl-CoA by cyclohexanoyl-CoA- and cyclohex-1-ene-1-carboxyl-CoA dehydrogenase activities Geobacter metallireducens