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Literature summary for 1.3.7.6 extracted from

  • Busch, A.W.; Reijerse, E.J.; Lubitz, W.; Frankenberg-Dinkel, N.; Hofmann, E.
    Structural and mechanistic insight into the ferredoxin-mediated two-electron reduction of bilins (2011), Biochem. J., 439, 257-264.
    View publication on PubMed
Search for more literature for 1.3.7.6

Protein Variants

Protein Variants Comment Organism
D105E site-directed mutagenesis, the mutant catalyzes only the first reaction step, i.e. the formation of 15,16-dihydrobiliverdin, reaction of EC 1.3.7.2 Prochlorococcus phage P-SSM2
D105N site-directed mutagenesis, inactive mutant Prochlorococcus phage P-SSM2
D206E site-directed mutagenesis, the mutant catalyzes only the second reaction step, i.e. the formation of (3Z)-phycoerythrobilin from 15,16-dihydrobiliverdin, reaction of EC 1.3.7.3 Prochlorococcus phage P-SSM2
D206N site-directed mutagenesis, the mutant catalyzes only the first reaction step, i.e. the formation of 15,16-dihydrobiliverdin, reaction of EC 1.3.7.2 Prochlorococcus phage P-SSM2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biliverdin IXalpha + 2 reduced ferredoxin Prochlorococcus phage P-SSM2 a two-step reaction via intermediate 15,16-dihydrobiliverdin, the single steps form the reactions of EC 1.3.7.2 and 1.3.7.3, overview (3Z)-phycoerythrobilin + 2 oxidized ferredoxin
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 ?

Organism

Organism UniProt Comment Textmining
Prochlorococcus phage P-SSM2
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biliverdin IXalpha + 2 reduced ferredoxin
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 Prochlorococcus phage P-SSM2 (3Z)-phycoerythrobilin + 2 oxidized ferredoxin
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 ?
biliverdin IXalpha + 2 reduced ferredoxin a two-step reaction via intermediate 15,16-dihydrobiliverdin, the single steps form the reactions of EC 1.3.7.2 and 1.3.7.3, overview Prochlorococcus phage P-SSM2 (3Z)-phycoerythrobilin + 2 oxidized ferredoxin
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 ?

Synonyms

Synonyms Comment Organism
PEB synthase
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 Prochlorococcus phage P-SSM2
PebS
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 Prochlorococcus phage P-SSM2
phycoerythrobilin synthase
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 Prochlorococcus phage P-SSM2

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
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 Prochlorococcus phage P-SSM2

General Information

General Information Comment Organism
evolution the enzyme belongs to the ferredoxin-dependent bilin reductase family Prochlorococcus phage P-SSM2
metabolism a two-step reaction via intermediate 15,16-dihydrobiliverdin, the single steps form the reactions of EC 1.3.7.2 and 1.3.7.3, overview Prochlorococcus phage P-SSM2
additional information aspartate residues Asp105 and Asp206 are both involved in interactions with the pyrrole nitrogens upon substrate binding. Both are essential for the complete reduction of biliverdin IXalpha to (3Z)-phycoerythrobilin by PebS and are highly conserved throughout the family of ferredoxin-dependent bilin reductases Prochlorococcus phage P-SSM2