Cloned (Comment) | Organism |
---|---|
PcyA expression in Escherichia coli strain BL21(DE3) | Thermosynechococcus vestitus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, recombinant enzyme, SDS-PAGE | Thermosynechococcus vestitus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin Ixalpha + reduced ferredoxin | Thermosynechococcus vestitus | - |
(3Z)-phycocyanobilin + oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermosynechococcus vestitus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion-exchange chromatography, hydrophobic interaction chromatography, hydroxyapatite chromatography, and gel filtration | Thermosynechococcus vestitus |
Storage Stability | Organism |
---|---|
-30°C, purified recombinant enzyme, 50 mM HEPES-NaOH, pH 7.5, containing 100 mM NaCl, 6 months, full activity is maintained | Thermosynechococcus vestitus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin Ixalpha + reduced ferredoxin | - |
Thermosynechococcus vestitus | (3Z)-phycocyanobilin + oxidized ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, recombinant enzyme, SDS-PAGE | Thermosynechococcus vestitus |
Synonyms | Comment | Organism |
---|---|---|
Pcb:Fd oxidoreductase | - |
Thermosynechococcus vestitus |
PcyA | - |
Thermosynechococcus vestitus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Thermosynechococcus vestitus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Thermosynechococcus vestitus |
General Information | Comment | Organism |
---|---|---|
metabolism | PcyA is involved in the phycocyanobilin biosynthetic pathway, in which PcyA first forms a stable complexes with the biliverdin IXa molecule | Thermosynechococcus vestitus |