BRENDA - Reference to 1.3.7.5; Id = 724317

recombinant functional expression of PcyA in Escherichia coli with production of phycocyanobilin, functional co-expression with cyanobacterial heme oxygenase, and the phycocyanin alpha-subunit, CpcA, from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002, and with the phycocyanin alpha-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin alpha-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Production levels of fluorescent pigments and chromophore analysis, overview

Synechococcus sp.

biliverdin Ixalpha + reduced ferredoxin

Synechococcus sp.

-

(3Z)-phycocyanobilin + oxidized ferredoxin

-

?

biliverdin Ixalpha + reduced ferredoxin

Synechococcus sp. 7002

-

(3Z)-phycocyanobilin + oxidized ferredoxin

-

?

Synechococcus sp.

-

Synechococcus sp. 7002

-

biliverdin Ixalpha + reduced ferredoxin

-

724317

Synechococcus sp.

(3Z)-phycocyanobilin + oxidized ferredoxin

-

?

biliverdin Ixalpha + reduced ferredoxin

-

724317

Synechococcus sp. 7002

(3Z)-phycocyanobilin + oxidized ferredoxin

-

?

3Z-phycocyanobilin:ferredoxin oxidoreductase

-

Synechococcus sp.

PcyA

-

Synechococcus sp.

Ferredoxin

-

Synechococcus sp.

recombinant functional expression of PcyA in Escherichia coli with production of phycocyanobilin, functional co-expression with cyanobacterial heme oxygenase, and the phycocyanin alpha-subunit, CpcA, from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002, and with the phycocyanin alpha-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin alpha-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Production levels of fluorescent pigments and chromophore analysis, overview

Synechococcus sp.

Ferredoxin

-

Synechococcus sp.

biliverdin Ixalpha + reduced ferredoxin

Synechococcus sp.

-

(3Z)-phycocyanobilin + oxidized ferredoxin

-

?

biliverdin Ixalpha + reduced ferredoxin

Synechococcus sp. 7002

-

(3Z)-phycocyanobilin + oxidized ferredoxin

-

?

biliverdin Ixalpha + reduced ferredoxin

-

724317

Synechococcus sp.

(3Z)-phycocyanobilin + oxidized ferredoxin

-

?

biliverdin Ixalpha + reduced ferredoxin

-

724317

Synechococcus sp. 7002

(3Z)-phycocyanobilin + oxidized ferredoxin

-

?

additional information

cyanobacteria utilize phycocyanobilin:ferredoxin oxidoreductase (PcyA) to perform a two-step reaction, the enzyme first reduces the 18-vinyl side chain of the D-ring and subsequently reduces the vinyl side chain of the pyrrole A ring to yield phycocyanobilin

Synechococcus sp.

additional information

cyanobacteria utilize phycocyanobilin:ferredoxin oxidoreductase (PcyA) to perform a two-step reaction, the enzyme first reduces the 18-vinyl side chain of the D-ring and subsequently reduces the vinyl side chain of the pyrrole A ring to yield phycocyanobilin

Synechococcus sp.

742318

Ceron-Carrasco

-

First computational step towa ...

Synechocystis sp. PCC 6803

Comput. Theoret. Chem.

1077

58-64

2016

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1

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1

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742524

Hagiwara

Atomic-resolution structure o ...

Synechocystis sp. PCC 6803

FEBS Lett.

590

3425-3434

2016

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1

-

2

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1

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1

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1

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724131

Kabasakal

His74 conservation in the bili ...

Synechocystis sp.

Arch. Biochem. Biophys.

537

233-242

2013

-

1

3

-

1

-

1

-

1

-

2

1

2

1

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1

-

1

-

1

3

-

1

-

1

-

2

1

-

1

-

3

-

724317

Alvey

Attachment of noncognate chrom ...

Synechococcus sp., Synechococcus sp. 7002

Biochemistry

50

4890-4902

2011

-

1

-

2

-

10

-

2

-

2

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1

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1

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2

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2

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1

-

725252

Alvey

Effects of modified phycobilin ...

Synechococcus sp.

J. Bacteriol.

193

1663-1671

2011

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1

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2

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4

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4

-

3

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1

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1

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1

-

2

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4

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3

-

711117

Wada

One residue substitution in Pc ...

Synechocystis sp.

Biochem. Biophys. Res. Commun.

402

373-377

2010

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1

-

1

-

3

-

2

-

1

-

1

-

1

-

1

-

2

-

712411

Hagiwara

Structural insights into vinyl ...

Synechocystis sp.

J. Biol. Chem.

285

1000-1007

2010

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1

-

1

-

5

-

3

-

1

-

1

-

1

-

1

-

1

-

3

-

1

-

1

-

724287

Shang

Biliverdin amides reveal roles ...

Anabaena sp., Anabaena sp. PCC 7120

Biochemistry

49

6070-6082

2010

-

1

-

2

-

20

-

1

-

13

-

2

-

2

-

1

-

1

-

2

-

1

-

13

-

2

-

2

-

724288

Kohler

Structural basis for hydration ...

Synechocystis sp.

Biochemistry

49

6206-6218

2010

-

1

2

-

1

-

3

-

1

-

1

-

1

-

1

2

-

1

-

1

-

1

-

2

-

696870

Zhang

Biosynthesis of fluorescent al ...

Synechocystis sp.

Biotechnol. Appl. Biochem.

52

135-140

2009

-

1

-

1

-

1

-

1

-

1

-

698489

Stoll

Structure of the biliverdin ra ...

Nostoc sp. PCC 7120 = FACHB-418, Synechocystis sp.

J. Am. Chem. Soc.

131

1986-1995

2009

-

2

3

-

7

-

2

3

-

712008

Okada

HO1 and PcyA proteins involved ...

Thermosynechococcus elongatus

FEBS Lett.

583

1251-1256

2009

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1

-

1

-

1

-

1

-

1

2

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

685125

Tu

Insight into the radical mecha ...

Nostoc sp. PCC 7120 = FACHB-418

Biochemistry

46

1484-1494

2007

-

1

5

-

4

-

1

-

1

-

2

-

1

5

-

1

-

1

-

673636

Mukougawa

Metabolic engineering to produ ...

Synechococcus sp., Synechococcus sp. W8020

FEBS Lett.

580

1333-1338

2006

-

1

-

2

-

2

-

1

-

1

-

2

-

673657

Hagiwara

Induced-fitting and electrosta ...

Synechocystis sp.

FEBS Lett.

580

3823-3828

2006

-

1

-

4

-

1

-

1

-

674669

Tu

A conserved histidine-aspartat ...

Nostoc sp., Nostoc sp. PCC 7120

J. Biol. Chem.

281

3127-3136

2006

-

1

-

16

-

1

-

15

-

1

-

1

2

-

1

-

1

-

16

-

1

-

1

-

1

2

-

674771

Zhao

Chromophore attachment to phyc ...

Anabaena sp., Anabaena sp. PCC 7120

J. Biol. Chem.

281

8573-8581

2006

-

1

-

18

-

2

-

1

-

1

-

2

-

676847

Hagiwara

Crystal structure of phycocyan ...

Synechocystis sp.

Proc. Natl. Acad. Sci. USA

103

27-32

2006

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

655796

Tu

Biliverdin reduction by cyanob ...

Anabaena sp.

J. Am. Chem. Soc.

126

8682-8693

2004

-

1

-

1

-

2

-

1

-

5

-

1

-

1

-

1

-

1

-

5

-

657164

Kami

Complementation of phytochrome ...

Synechocystis sp.

Proc. Natl. Acad. Sci. USA

101

1099-1104

2004

-

1

-

1

-

3

-

1

-

1

-

1

-

1

-

1

-

656197

Frankenberg

Phycocyanobilin:ferredoxin oxi ...

Anabaena sp.

J. Biol. Chem.

278

9219-9226

2003

-

1

-

2

1

-

5

-

4

1

-

1

-

2

1

-

4

1

-

437763

Tooley

Biosynthesis of the cyanobacte ...

Anabaena sp.

J. Bacteriol.

184

4666-4671

2002

-

1

-

1

-

1

-

1

-

1

-

437673

Frankenberg

Functional genomic analysis of ...

Anabaena sp., Nostoc punctiforme, Prochlorococcus sp., Prochlorococcus sp. CCMP1378 / MED4, Synechococcus sp., Synechococcus sp. WH8102, Synechocystis sp.

Plant Cell

13

965-978

2001

-

5

-

2

-

8

-

5

-

7

-

5

-

2

-

5

-

7

-

437764

Tooley

Biosynthesis of a fluorescent ...

Synechocystis sp.

Proc. Natl. Acad. Sci. USA

98

10560-10565

2001

-

1

-

4

-

1

-

1

-

1

-

437765

Wu

Phycocyanobilin is the natural ...

Mesotaenium caldariorum

J. Biol. Chem.

272

25700-25705

1997

-

1

-

1

-

1

-

1

-

1

-

Attachment of noncognate chromophores to CpcA of Synechocystis sp. PCC 6803 and Synechococcus sp. PCC 7002 by heterologous expression in Escherichia coli

Data extracted from this reference: