BRENDA - Enzyme Database
show all sequences of 1.3.7.5

A conserved histidine-aspartate pair is required for exovinyl reduction of biliverdin by a cyanobacterial phycocyanobilin:ferredoxin oxidoreductase

Tu, S.L.; Sughrue, W.; Britt, R.D.; Lagarias, J.C.; J. Biol. Chem. 281, 3127-3136 (2006)

Data extracted from this reference:

Application
Application
Commentary
Organism
additional information
conserved histidine and aspartate residues essential for the catalytic activity of the enzyme, direct role of the His85-Asp102 pair in exovinyl reduction of biliverdin IXa
Nostoc sp.
Cloned(Commentary)
Cloned (Commentary)
Organism
wild-type and mutants, mutant plasmid constructs expressed in Escherichia coli strain DH5alpha
Nostoc sp.
Engineering
Protein Variants
Commentary
Organism
C210A
retains 90% relative to wild type
Nostoc sp.
C86A
retains 90% activity relative to wild type
Nostoc sp.
D102N
retains 11% activity relative to wild type
Nostoc sp.
D116N
retains 11% activity relative to wild type
Nostoc sp.
D217N
retains 87% relative to wild type
Nostoc sp.
H71A
retains less than 1% activity relative to wild type
Nostoc sp.
H71D
retains 28% activity relative to wild type
Nostoc sp.
H71E
retains 50% activity relative to wild type
Nostoc sp.
H71N
retains 46% activity relative to wild type
Nostoc sp.
H71Q
retains 65% activity relative to wild type
Nostoc sp.
H85A
retains less than 1% activity relative to wild type
Nostoc sp.
H85D
retains less than 1% activity relative to wild type
Nostoc sp.
H85E
retains 5% activity relative to wild type
Nostoc sp.
H85N
retains less than 1% activity relative to wild type
Nostoc sp.
H85Q
retains 5% activity relative to wild type
Nostoc sp.
K218E
retains 20% activity relative to wild type
Nostoc sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
diethylpyrocarbonate
reduces PcyA activity to less than 20% of mock-treated enzyme within 10 min, diethylpyrocarbonate-inactivation can be prevented by the presence of biliverdin IXa substrate
Nostoc sp.
Organism
Organism
UniProt
Commentary
Textmining
Nostoc sp.
-
-
-
Nostoc sp. PCC 7120
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
wild-type and mutants, histidine mutants purified to homogeneity
Nostoc sp.
Storage Stability
Storage Stability
Organism
-80°C
Nostoc sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
biliverdin IXa + reduced ferredoxin
-
674669
Nostoc sp.
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
-
?
biliverdin IXa + reduced ferredoxin
-
674669
Nostoc sp. PCC 7120
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
PcyA
-
Nostoc sp.
Application (protein specific)
Application
Commentary
Organism
additional information
conserved histidine and aspartate residues essential for the catalytic activity of the enzyme, direct role of the His85-Asp102 pair in exovinyl reduction of biliverdin IXa
Nostoc sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
wild-type and mutants, mutant plasmid constructs expressed in Escherichia coli strain DH5alpha
Nostoc sp.
Engineering (protein specific)
Protein Variants
Commentary
Organism
C210A
retains 90% relative to wild type
Nostoc sp.
C86A
retains 90% activity relative to wild type
Nostoc sp.
D102N
retains 11% activity relative to wild type
Nostoc sp.
D116N
retains 11% activity relative to wild type
Nostoc sp.
D217N
retains 87% relative to wild type
Nostoc sp.
H71A
retains less than 1% activity relative to wild type
Nostoc sp.
H71D
retains 28% activity relative to wild type
Nostoc sp.
H71E
retains 50% activity relative to wild type
Nostoc sp.
H71N
retains 46% activity relative to wild type
Nostoc sp.
H71Q
retains 65% activity relative to wild type
Nostoc sp.
H85A
retains less than 1% activity relative to wild type
Nostoc sp.
H85D
retains less than 1% activity relative to wild type
Nostoc sp.
H85E
retains 5% activity relative to wild type
Nostoc sp.
H85N
retains less than 1% activity relative to wild type
Nostoc sp.
H85Q
retains 5% activity relative to wild type
Nostoc sp.
K218E
retains 20% activity relative to wild type
Nostoc sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
diethylpyrocarbonate
reduces PcyA activity to less than 20% of mock-treated enzyme within 10 min, diethylpyrocarbonate-inactivation can be prevented by the presence of biliverdin IXa substrate
Nostoc sp.
Purification (Commentary) (protein specific)
Commentary
Organism
wild-type and mutants, histidine mutants purified to homogeneity
Nostoc sp.
Storage Stability (protein specific)
Storage Stability
Organism
-80°C
Nostoc sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
biliverdin IXa + reduced ferredoxin
-
674669
Nostoc sp.
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
-
?
biliverdin IXa + reduced ferredoxin
-
674669
Nostoc sp. PCC 7120
(3Z)-phycocyanobilin + oxidized ferredoxin
-
-
-
?
Other publictions for EC 1.3.7.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742318
Ceron-Carrasco
-
First computational step towa ...
Synechocystis sp. PCC 6803
Comput. Theoret. Chem.
1077
58-64
2016
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742524
Hagiwara
Atomic-resolution structure o ...
Synechocystis sp. PCC 6803
FEBS Lett.
590
3425-3434
2016
-
-
1
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724131
Kabasakal
His74 conservation in the bili ...
Synechocystis sp.
Arch. Biochem. Biophys.
537
233-242
2013
-
-
1
1
3
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
1
2
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
724317
Alvey
Attachment of noncognate chrom ...
Synechococcus sp., Synechococcus sp. 7002
Biochemistry
50
4890-4902
2011
-
-
1
-
-
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725252
Alvey
Effects of modified phycobilin ...
Synechococcus sp.
J. Bacteriol.
193
1663-1671
2011
-
-
-
-
1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
711117
Wada
One residue substitution in Pc ...
Synechocystis sp.
Biochem. Biophys. Res. Commun.
402
373-377
2010
-
-
-
1
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712411
Hagiwara
Structural insights into vinyl ...
Synechocystis sp.
J. Biol. Chem.
285
1000-1007
2010
-
-
-
1
1
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
724287
Shang
Biliverdin amides reveal roles ...
Anabaena sp., Anabaena sp. PCC 7120
Biochemistry
49
6070-6082
2010
-
-
1
-
-
-
-
-
-
-
-
2
-
20
-
-
1
-
-
-
-
-
13
-
2
-
-
-
-
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
13
-
-
-
-
-
2
-
-
-
-
2
2
-
-
-
724288
Kohler
Structural basis for hydration ...
Synechocystis sp.
Biochemistry
49
6206-6218
2010
-
-
1
1
2
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
696870
Zhang
Biosynthesis of fluorescent al ...
Synechocystis sp.
Biotechnol. Appl. Biochem.
52
135-140
2009
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698489
Stoll
Structure of the biliverdin ra ...
Nostoc sp. PCC 7120 = FACHB-418, Synechocystis sp.
J. Am. Chem. Soc.
131
1986-1995
2009
-
-
-
2
3
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712008
Okada
HO1 and PcyA proteins involved ...
Thermosynechococcus elongatus
FEBS Lett.
583
1251-1256
2009
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
1
1
1
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
1
1
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
685125
Tu
Insight into the radical mecha ...
Nostoc sp. PCC 7120 = FACHB-418
Biochemistry
46
1484-1494
2007
-
-
-
1
5
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
5
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673636
Mukougawa
Metabolic engineering to produ ...
Synechococcus sp., Synechococcus sp. W8020
FEBS Lett.
580
1333-1338
2006
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673657
Hagiwara
Induced-fitting and electrosta ...
Synechocystis sp.
FEBS Lett.
580
3823-3828
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674669
Tu
A conserved histidine-aspartat ...
Nostoc sp., Nostoc sp. PCC 7120
J. Biol. Chem.
281
3127-3136
2006
-
1
1
-
16
-
1
-
-
-
-
-
-
15
-
-
1
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
16
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674771
Zhao
Chromophore attachment to phyc ...
Anabaena sp., Anabaena sp. PCC 7120
J. Biol. Chem.
281
8573-8581
2006
-
1
1
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676847
Hagiwara
Crystal structure of phycocyan ...
Synechocystis sp.
Proc. Natl. Acad. Sci. USA
103
27-32
2006
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655796
Tu
Biliverdin reduction by cyanob ...
Anabaena sp.
J. Am. Chem. Soc.
126
8682-8693
2004
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
5
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657164
Kami
Complementation of phytochrome ...
Synechocystis sp.
Proc. Natl. Acad. Sci. USA
101
1099-1104
2004
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656197
Frankenberg
Phycocyanobilin:ferredoxin oxi ...
Anabaena sp.
J. Biol. Chem.
278
9219-9226
2003
-
-
1
-
-
-
-
-
-
-
2
1
-
5
-
-
-
-
-
-
-
-
4
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437763
Tooley
Biosynthesis of the cyanobacte ...
Anabaena sp.
J. Bacteriol.
184
4666-4671
2002
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437673
Frankenberg
Functional genomic analysis of ...
Anabaena sp., Nostoc punctiforme, Prochlorococcus sp., Prochlorococcus sp. CCMP1378 / MED4, Synechococcus sp., Synechococcus sp. WH8102, Synechocystis sp.
Plant Cell
13
965-978
2001
-
-
5
-
-
-
-
-
-
-
2
-
-
8
-
-
5
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
5
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437764
Tooley
Biosynthesis of a fluorescent ...
Synechocystis sp.
Proc. Natl. Acad. Sci. USA
98
10560-10565
2001
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
437765
Wu
Phycocyanobilin is the natural ...
Mesotaenium caldariorum
J. Biol. Chem.
272
25700-25705
1997
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-