BRENDA - Enzyme Database
show all sequences of 1.3.7.4

Mechanistic studies of the phytochromobilin synthase HY2 from Arabidopsis

Tu, S.L.; Chen, H.C.; Ku, L.W.; J. Biol. Chem. 283, 27555-27564 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli strain BL21
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
D116N
mutant still retains the ability of substrate binding, but with only 1.5% relative activity of wild type protein
Arabidopsis thaliana
D146N
mutant completely loses catalytic activity and also the ability of biliverdin binding
Arabidopsis thaliana
D256E
mutant retains only partial activity
Arabidopsis thaliana
N133
mutant produces only partial activity
Arabidopsis thaliana
R252Q
mutant loses catalytic activity and the ability of substrate binding
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Purification (Commentary)
Commentary
Organism
Superdex 200 gel filtration
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
Ferredoxin
dependent
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli strain BL21
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Ferredoxin
dependent
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D116N
mutant still retains the ability of substrate binding, but with only 1.5% relative activity of wild type protein
Arabidopsis thaliana
D146N
mutant completely loses catalytic activity and also the ability of biliverdin binding
Arabidopsis thaliana
D256E
mutant retains only partial activity
Arabidopsis thaliana
N133
mutant produces only partial activity
Arabidopsis thaliana
R252Q
mutant loses catalytic activity and the ability of substrate binding
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
Superdex 200 gel filtration
Arabidopsis thaliana
Other publictions for EC 1.3.7.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742513
Shin
Expression of recombinant ful ...
Arabidopsis thaliana
FEBS Lett.
588
2964-2970
2014
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1
1
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1
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6
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724317
Alvey
Attachment of noncognate chrom ...
Arabidopsis thaliana
Biochemistry
50
4890-4902
2011
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1
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1
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8
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1
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725252
Alvey
Effects of modified phycobilin ...
Arabidopsis thaliana
J. Bacteriol.
193
1663-1671
2011
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712484
Chiu
Electrostatic interaction of p ...
Arabidopsis thaliana
J. Biol. Chem.
285
5056-5065
2010
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9
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687760
Tu
Mechanistic studies of the phy ...
Arabidopsis thaliana
J. Biol. Chem.
283
27555-27564
2008
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5
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685125
Tu
Insight into the radical mecha ...
Arabidopsis thaliana
Biochemistry
46
1484-1494
2007
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673636
Mukougawa
Metabolic engineering to produ ...
Escherichia coli
FEBS Lett.
580
1333-1338
2006
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743476
Creff
Targeted Ds-tagging strategy ...
Arabidopsis thaliana
Plant Mol. Biol.
61
603-613
2006
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1
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676460
Muramoto
The tomato photomorphogenetic ...
Solanum lycopersicum
Plant Cell Physiol.
46
661-665
2005
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657069
Sawers
The Elm1 (ZmHy2) gene of maize ...
Zea mays
Plant Physiol.
136
2771-2781
2004
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1
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5
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437673
Frankenberg
Functional genomic analysis of ...
Arabidopsis thaliana
Plant Cell
13
965-978
2001
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1
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6
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437767
Kohchi
The Arabidopsis hy2 gene encod ...
Arabidopsis thaliana
Plant Cell
13
425-436
2001
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437768
McDowell
Purification and biochemical p ...
Avena sativa
Plant Physiol.
126
1546-1554
2001
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3
1
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437769
Terry
Holophytochrome assembly. Coup ...
Cucumis sativus
J. Biol. Chem.
266
22215-22221
1991
4
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