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Literature summary for 1.3.7.2 extracted from
- Insights into phycoerythrobilin biosynthesis point toward metabolic channeling (2006), J. Biol. Chem., 281, 27081-27089.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed using a tac promoter-driven N-terminal GST fusion protein | Synechococcus sp. |
| into pGEX-6P-3 vector for N-terminal fusion with glutathione S-transferase and transformed in Escherichia coli BL21(lambdaDE3) cells | Microchaete diplosiphon |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Microchaete diplosiphon | Q6UR88 | - |
- |
| Microchaete diplosiphon Fd33 | Q6UR88 | - |
- |
| Synechococcus sp. | - |
- |
- |
| Synechococcus sp. WH8020 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by two affinity chromatography steps to 90% purity and a third gel filtration step | Synechococcus sp. |
| by two affinity chromatography steps to 90% purity and a third gel filtration step | Microchaete diplosiphon |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 15,16-dihydrobiliverdin + reduced ferredoxin | - |
Microchaete diplosiphon | phycoerythrobilin + oxidized ferredoxin | - |
r |  |
| 15,16-dihydrobiliverdin + reduced ferredoxin | - |
Microchaete diplosiphon Fd33 | phycoerythrobilin + oxidized ferredoxin | - |
r | |
| biliverdin IXa + reduced ferredoxin | - |
Synechococcus sp. | 15,16-dihydrobiliverdin + oxidized ferredoxin | - |
r | |
| biliverdin IXa + reduced ferredoxin | - |
Microchaete diplosiphon | 15,16-dihydrobiliverdin + oxidized ferredoxin | - |
r | |
| biliverdin IXa + reduced ferredoxin | - |
Synechococcus sp. WH8020 | 15,16-dihydrobiliverdin + oxidized ferredoxin | - |
r | |
| biliverdin IXa + reduced ferredoxin | - |
Microchaete diplosiphon Fd33 | 15,16-dihydrobiliverdin + oxidized ferredoxin | - |
r | |
| additional information | does not convert 3E-phycocyanobilin | Microchaete diplosiphon | ? | - |
? | |
| additional information | does not convert phycocyanobilin | Synechococcus sp. | ? | - |
? | |
| additional information | does not convert phycocyanobilin | Synechococcus sp. WH8020 | ? | - |
? | |
| additional information | does not convert 3E-phycocyanobilin | Microchaete diplosiphon Fd33 | ? | - |
? | |
| phytochromobilin + biliverdin IXa + reduced ferredoxin | - |
Synechococcus sp. | phycoerythrobilin | - |
r | |
| phytochromobilin + biliverdin IXa + reduced ferredoxin | - |
Synechococcus sp. WH8020 | phycoerythrobilin | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PebA | - |
Synechococcus sp. |
| PebA | - |
Microchaete diplosiphon |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Microchaete diplosiphon |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | - |
Synechococcus sp. | |
| Ferredoxin | - |
Microchaete diplosiphon | |
| additional information | insensitivity toward metal chelators like 0.01 mM EDTA, 0.005 mM o-phenanthroline, or 0.005 mM 2,2'-dipyridyl | Microchaete diplosiphon | |
| NADP+ | - |
Synechococcus sp. | |
| NADP+ | - |
Microchaete diplosiphon | |
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Release 2023.1 (January 2023)
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