Literature summary for 1.3.7.15 extracted from

  • Yamamoto, H.; Mizoguchi, T.; Tsukatani, Y.; Tamiaki, H.; Kurisu, G.; Fujita, Y.
    Chlorophyllide a oxidoreductase preferentially catalyzes 8-vinyl reduction over B-ring reduction of 8-vinyl chlorophyllide a in the late steps of bacteriochlorophyll biosynthesis (2020), ChemBioChem, 21, 1760-1766 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genes bchX and bchY, recombinant expression of GST-tagged BchX and BchY in Escherichia coli Rhodobacter capsulatus

Inhibitors

Inhibitors Comment Organism Structure
8-vinyl chlorophyllide a inhibitory effect of 8 V-Chlide on 3 V-BChlide production inhibitory effect of 8 V-Chlide on the C7=C8 reduction of COR Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
8-vinyl chlorophyllide a recombinant enzyme BchX/BchY, pH and temperature not specified in the publication Rhodobacter capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ Rhodobacter capsulatus
-
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Rhodobacter capsulatus
-
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ Rhodobacter capsulatus NBRC 16581
-
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Rhodobacter capsulatus NBRC 16581
-
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ Rhodobacter capsulatus ATCC BAA-309
-
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Rhodobacter capsulatus ATCC BAA-309
-
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ Rhodobacter capsulatus SB1003
-
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Rhodobacter capsulatus SB1003
-
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus P26177 gene bchX
-
Rhodobacter capsulatus NBRC 16581 P26177 gene bchX
-
Rhodobacter capsulatus ATCC BAA-309 P26177 gene bchX
-
Rhodobacter capsulatus SB1003 P26177 gene bchX
-

Purification (Commentary)

Purification (Comment) Organism
copurification of recombinant GST-tagged BchX and BchY from Escherichia coli by glutathione affinity chromatography Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme COR has an alternative 8VR activity in addition to the B-ring C7=C8 double bond reduction activity, cf. EC 1.3.7.13 or EC 1.3.1.75 Rhodobacter capsulatus ?
-
-
additional information enzyme COR has an alternative 8VR activity in addition to the B-ring C7=C8 double bond reduction activity, cf. EC 1.3.7.13 or EC 1.3.1.75 Rhodobacter capsulatus NBRC 16581 ?
-
-
additional information enzyme COR has an alternative 8VR activity in addition to the B-ring C7=C8 double bond reduction activity, cf. EC 1.3.7.13 or EC 1.3.1.75 Rhodobacter capsulatus ATCC BAA-309 ?
-
-
additional information enzyme COR has an alternative 8VR activity in addition to the B-ring C7=C8 double bond reduction activity, cf. EC 1.3.7.13 or EC 1.3.1.75 Rhodobacter capsulatus SB1003 ?
-
-
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+
-
Rhodobacter capsulatus bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+
-
Rhodobacter capsulatus NBRC 16581 bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+
-
Rhodobacter capsulatus ATCC BAA-309 bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+
-
Rhodobacter capsulatus SB1003 bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
Rhodobacter capsulatus chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
Rhodobacter capsulatus NBRC 16581 chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
Rhodobacter capsulatus ATCC BAA-309 chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
Rhodobacter capsulatus SB1003 chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster
-
?

Subunits

Subunits Comment Organism
More In contrast to BciA and BciB, COR is a nitrogenase-like complex enzyme consisting of two separable components, the X protein (a BchX dimer) and the YZ protein (a BchY-BchZ heterotetramer). The X protein serves as the reductase component by transferring electrons from a [4Fe-4S] cluster held in the interface between the BchX homodimer to the YZ protein. The YZ protein is the catalytic component, as it accepts electrons from the X protein and transfers them to the Chlide substrate via a [4Fe-4S] cluster, to produce 3-vinyl bacteriochlorophyllide a (3 V-BChlide) Rhodobacter capsulatus

Synonyms

Synonyms Comment Organism
COR
-
Rhodobacter capsulatus
chlorophyllide a oxidoreductase
-
Rhodobacter capsulatus
COR 8VR
-
Rhodobacter capsulatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00027
-
8-vinyl chlorophyllide a recombinant enzyme BchX/BchY, pH and temperature not specified in the publication Rhodobacter capsulatus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Rhodobacter capsulatus
Ferredoxin
-
Rhodobacter capsulatus
Fe-S center a [4Fe-4S] cluster is bound at the interface between the BchX homodimer and the YZ protein of the COR enzyme complex Rhodobacter capsulatus

General Information

General Information Comment Organism
physiological function bacteriochlorophyll a (BChl) is an essential pigment for anoxygenic photosynthesis. In late steps of the BChl biosynthesis of Rhodobacter capsulatus, the C8 vinyl group and C7=C8 double bond of 8-vinyl chlorophyllide a (8 V-Chlide) are reduced by a C8 vinyl reductase (8VR), BciA (EC 1.3.1.75) or BciB (EC 1.3.7.13), and a nitrogenase-like enzyme, chlorophyllide a oxidoreductase (COR), respectively, to produce 3-vinyl-bacteriochlorphyllide a. The COR activity can act as a third 8VR, confirmed in vivo by the loss of 8VR activity mutants lacking bciA and genes encoding subunits of COR. In contrast to BciA and BciB, COR is a nitrogenase-like complex enzyme consisting of two separable components, the X protein (a BchX dimer) and the YZ protein (a BchY-BchZ heterotetramer). The X protein serves as the reductase component by transferring electrons from a [4Fe-4S] cluster held in the interface between the BchX homodimer and the YZ protein. The YZ protein is the catalytic component, as it accepts electrons from the X protein and transfers them to the Chlide substrate via a [4Fe-4S] cluster, to produce 3-vinyl bacteriochlorophyllide a (3 V-BChlide). 3 V-BChlide is produced via Chlide rather than via 3,8-divinyl BChlide (81,82-didehydro-3 V-BChlide) Rhodobacter capsulatus
metabolism bacteriochlorophyll a (BChl) is an essential pigment for anoxygenic photosynthesis. In late steps of the BChl biosynthesis of Rhodobacter capsulatus, the C8 vinyl group and C7=C8 double bond of 8-vinyl chlorophyllide a (8 V-Chlide) are reduced by a C8 vinyl reductase (8VR), BciA (EC 1.3.1.75) or BciB (EC 1.3.7.13), and a nitrogenase-like enzyme, chlorophyllide a oxidoreductase (COR), respectively, to produce 3-vinyl-bacteriochlorphyllide a. BciB is a FAD-containing FeS protein that uses reduced ferredoxin as the reductant for the 8-vinyl reduction of 8 V-Chlide or 8 V-PChlide, while BciA utilizes NADPH Rhodobacter capsulatus