Literature summary for 1.3.7.15 extracted from

Nomata, J.; Mizoguchi, T.; Tamiaki, H.; Fujita, Y.
A second nitrogenase-like enzyme for bacteriochlorophyll biosynthesis: reconstitution of chlorophyllide a reductase with purified X-protein (BchX) and YZ-protein (BchY-BchZ) from Rhodobacter capsulatus (2006), J. Biol. Chem., 281, 15021-15028.

Cloned(Commentary)

Cloned (Comment)	Organism
reconstitution of chlorophyllide a reductase with purified proteins. Two Rhodobacter capsulatus strains that overexpress Strep-tagged BchX and BchY are isolated. Strep-tagged BchX is purified as a single polypeptide, and BchZ is co-purified with Strep-tagged BchY. When BchX and BchY-BchZ components are incubated with chlorophyllide a, ATP, and dithionite under anaerobic conditions, chlorophyllide a is converted to a new pigment	Rhodobacter capsulatus

Cloned (Comment)

Organism

reconstitution of chlorophyllide a reductase with purified proteins. Two Rhodobacter capsulatus strains that overexpress Strep-tagged BchX and BchY are isolated. Strep-tagged BchX is purified as a single polypeptide, and BchZ is co-purified with Strep-tagged BchY. When BchX and BchY-BchZ components are incubated with chlorophyllide a, ATP, and dithionite under anaerobic conditions, chlorophyllide a is converted to a new pigment

Rhodobacter capsulatus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
[4Fe-4S]-center	-	Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
chlorophyllide a + AH2 + ATP + H2O	Rhodobacter capsulatus	-	3-deacetyl-3-vinylbacteriochlorophyllide a + A + ADP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Rhodobacter capsulatus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chlorophyllide a + AH2 + ATP + H2O	-	Rhodobacter capsulatus	3-deacetyl-3-vinylbacteriochlorophyllide a + A + ADP + phosphate	-	?
chlorophyllide a + AH2 + ATP + H2O	the enzyme catalyses trans-reduction of the B-ring of chlorophyllide a. The product has the (7R,8R)-configuration	Rhodobacter capsulatus	3-deacetyl-3-vinylbacteriochlorophyllide a + A + ADP + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
BchX	-	Rhodobacter capsulatus
BchY	-	Rhodobacter capsulatus
BchZ	-	Rhodobacter capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
34	-	assay at	Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Rhodobacter capsulatus