BRENDA - Enzyme Database
show all sequences of 1.3.7.13

Evolution of a new chlorophyll metabolic pathway driven by the dynamic changes in enzyme promiscuous activity

Ito, H.; Tanaka, A.; Plant Cell Physiol. 55, 593-603 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene slr1923, phylogenetic analysis
Synechocystis sp. PCC 6803
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3,8-divinyl chlorophyll a + reduced ferredoxin [iron-sulfur] cluster + H+
Synechocystis sp. PCC 6803
-
chlorophyll a + oxidized ferredoxin [iron-sulfur] cluster
-
-
?
3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
Synechocystis sp. PCC 6803
-
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
-
-
?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
Synechocystis sp. PCC 6803
-
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
-
-
?
additional information
Synechocystis sp. PCC 6803
cyanobacterial 3,8-divinyl chlorophyllide reductase (DVR) is a Chl biosynthetic enzyme that has many promiscuous activities besides the 3,8-divinyl chlorophyllide reductase activity, e.g. 7-hydroxymethyl chlorophyll a reductase activity. The Chl c2 to Chl c1 conversion is existent in cyanobacteria as a promiscuous activity of F-DVR. Chl c1 is synthesized by the reduction of the 8-vinyl group of Chl c2 or Chl c1 is synthesized from monovinyl-protochlorophyllide by the dehydrogenase
?
-
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-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Synechocystis sp. PCC 6803
P74473
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,8-divinyl chlorophyll a + reduced ferredoxin [iron-sulfur] cluster + H+
-
746037
Synechocystis sp. PCC 6803
chlorophyll a + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
-
746037
Synechocystis sp. PCC 6803
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
-
746037
Synechocystis sp. PCC 6803
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
additional information
cyanobacterial 3,8-divinyl chlorophyllide reductase (DVR) is a Chl biosynthetic enzyme that has many promiscuous activities besides the 3,8-divinyl chlorophyllide reductase activity, e.g. 7-hydroxymethyl chlorophyll a reductase activity. The Chl c2 to Chl c1 conversion is existent in cyanobacteria as a promiscuous activity of F-DVR. Chl c1 is synthesized by the reduction of the 8-vinyl group of Chl c2 or Chl c1 is synthesized from monovinyl-protochlorophyllide by the dehydrogenase
746037
Synechocystis sp. PCC 6803
?
-
-
-
-
additional information
substrate specificity, overview. Cyanobacterial F-DVR exhibits a broad substrate specificity and converts 3,8-divinylprotochlorophyllide a, 3,8-divinyl chlorophyllide a, and 3,8-divinyl chlorophyll a to the respective monovinyl molecules. When 7-hydroxymethyl chlorophyllide (HMChlide) a is incubated with F-DVR, chlorophyllide a is generated
746037
Synechocystis sp. PCC 6803
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
ferredoxin [iron-sulfur] cluster
dependent on
Synechocystis sp. PCC 6803
Cloned(Commentary) (protein specific)
Commentary
Organism
gene slr1923, phylogenetic analysis
Synechocystis sp. PCC 6803
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ferredoxin [iron-sulfur] cluster
dependent on
Synechocystis sp. PCC 6803
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3,8-divinyl chlorophyll a + reduced ferredoxin [iron-sulfur] cluster + H+
Synechocystis sp. PCC 6803
-
chlorophyll a + oxidized ferredoxin [iron-sulfur] cluster
-
-
?
3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
Synechocystis sp. PCC 6803
-
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
-
-
?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
Synechocystis sp. PCC 6803
-
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
-
-
?
additional information
Synechocystis sp. PCC 6803
cyanobacterial 3,8-divinyl chlorophyllide reductase (DVR) is a Chl biosynthetic enzyme that has many promiscuous activities besides the 3,8-divinyl chlorophyllide reductase activity, e.g. 7-hydroxymethyl chlorophyll a reductase activity. The Chl c2 to Chl c1 conversion is existent in cyanobacteria as a promiscuous activity of F-DVR. Chl c1 is synthesized by the reduction of the 8-vinyl group of Chl c2 or Chl c1 is synthesized from monovinyl-protochlorophyllide by the dehydrogenase
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,8-divinyl chlorophyll a + reduced ferredoxin [iron-sulfur] cluster + H+
-
746037
Synechocystis sp. PCC 6803
chlorophyll a + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
3,8-divinyl chlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
-
746037
Synechocystis sp. PCC 6803
chlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
-
746037
Synechocystis sp. PCC 6803
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
-
-
-
?
additional information
cyanobacterial 3,8-divinyl chlorophyllide reductase (DVR) is a Chl biosynthetic enzyme that has many promiscuous activities besides the 3,8-divinyl chlorophyllide reductase activity, e.g. 7-hydroxymethyl chlorophyll a reductase activity. The Chl c2 to Chl c1 conversion is existent in cyanobacteria as a promiscuous activity of F-DVR. Chl c1 is synthesized by the reduction of the 8-vinyl group of Chl c2 or Chl c1 is synthesized from monovinyl-protochlorophyllide by the dehydrogenase
746037
Synechocystis sp. PCC 6803
?
-
-
-
-
additional information
substrate specificity, overview. Cyanobacterial F-DVR exhibits a broad substrate specificity and converts 3,8-divinylprotochlorophyllide a, 3,8-divinyl chlorophyllide a, and 3,8-divinyl chlorophyll a to the respective monovinyl molecules. When 7-hydroxymethyl chlorophyllide (HMChlide) a is incubated with F-DVR, chlorophyllide a is generated
746037
Synechocystis sp. PCC 6803
?
-
-
-
-
General Information
General Information
Commentary
Organism
evolution
either F-DVR or N-DVR is found in most photosynthetic organisms, yet both F-DVR and N-DVR exist in the genome of diatoms that contain Chl a and Chl c1
Synechocystis sp. PCC 6803
metabolism
the enzyme is involved the chlorophyll biosynthetic pathways of oxygenic photosynthetic organisms. At the later steps of chlorophyll biosynthesis, 3,8-divinyl-chlorophyllide (Chlide) a is converted to monovinyl (MV)-Chlide a by DVR. Involvement of DVR in Chl c biosynthesis
Synechocystis sp. PCC 6803
General Information (protein specific)
General Information
Commentary
Organism
evolution
either F-DVR or N-DVR is found in most photosynthetic organisms, yet both F-DVR and N-DVR exist in the genome of diatoms that contain Chl a and Chl c1
Synechocystis sp. PCC 6803
metabolism
the enzyme is involved the chlorophyll biosynthetic pathways of oxygenic photosynthetic organisms. At the later steps of chlorophyll biosynthesis, 3,8-divinyl-chlorophyllide (Chlide) a is converted to monovinyl (MV)-Chlide a by DVR. Involvement of DVR in Chl c biosynthesis
Synechocystis sp. PCC 6803
Other publictions for EC 1.3.7.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738542
Chen
Two unrelated 8-vinyl reductas ...
Acaryochloris marina
J. Bacteriol.
198
1393-1400
2016
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1
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1
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1
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745221
Chen
Two unrelated 8-vinyl reducta ...
Acaryochloris marina, Acaryochloris marina MBIC11017
J. Bacteriol.
198
1393-1400
2016
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1
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2
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2
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1
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2
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1
2
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2
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1
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4
4
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728467
Ito
Evolution of a new chlorophyll ...
Synechocystis sp.
Plant Cell Physiol.
55
593-603
2014
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1
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736444
Harada
Chlorophyllide a oxidoreductas ...
no activity in Rhodobacter sphaeroides, Rhodopseudomonas palustris, Rhodopseudomonas palustris J1002
J. Biol. Chem.
289
12716-12726
2014
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3
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737646
Canniffe
Elucidation of the preferred r ...
Synechocystis sp.
Biochem. J.
462
433-440
2014
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744270
Canniffe
Elucidation of the preferred ...
Synechocystis sp. PCC 6803
Biochem. J.
462
433-440
2014
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1
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3
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746037
Ito
Evolution of a new chlorophyl ...
Synechocystis sp. PCC 6803
Plant Cell Physiol.
55
593-603
2014
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1
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4
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1
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2
2
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737687
Saunders
Characterization of BciB: a fe ...
Chloroherpeton thalassium
Biochemistry
52
8442-8451
2013
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1
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1
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8
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744314
Saunders
Characterization of BciB a fe ...
Chloroherpeton thalassium, Chloroherpeton thalassium ATCC 35110
Biochemistry
52
8442-8451
2013
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1
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9
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4
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1
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738520
Liu
Multiple types of 8-vinyl redu ...
Chlorobium ferrooxidans, Chlorobium ferrooxidans DSM 13031, Chlorobium limicola, Chlorobium limicola DSM 245, Chlorobium phaeobacteroides, Chlorobium phaeobacteroides DSM 266, Chloroherpeton thalassium, Chloroherpeton thalassium ATCC 35110, Pelodictyon clathratiforme, Pelodictyon clathratiforme DSM 5477, Prosthecochloris aestuarii, Prosthecochloris aestuarii DSM 271
J. Bacteriol.
193
4996-4998
2011
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