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Literature summary for 1.3.7.11 extracted from

  • Ogawa, T.; Isobe, K.; Mori, T.; Asakawa, S.; Yoshimura, T.; Hemmi, H.
    A novel geranylgeranyl reductase from the methanogenic archaeon Methanosarcinaacetivorans displays unique regiospecificity (2014), FEBS J., 281, 3165-3176 .
    View publication on PubMed
Search for more literature for 1.3.7.11

Cloned(Commentary)

Cloned (Comment) Organism
gene MA_1492, sequence comparisons, recombinant expression of N-terminally poly-His-tagged enzyme MA1492 in Escherichia coli KRX cells, which are genetically modified to produce unsaturated archaeal-type lipids, leads to production of partially saturated lipid derivatives Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 2,3-bis-(O-phytanyl)-sn-glycero-phospholipid + 16 oxidized ferredoxin [iron-sulfur] cluster Methanosarcina acetivorans
-
 a 2,3-bis-(O-geranylgeranyl)-sn-glycero-phospholipid + 16 reduced ferredoxin [iron-sulfur] cluster + 16 H+
-
 ?
a 2,3-bis-(O-phytanyl)-sn-glycero-phospholipid + 16 oxidized ferredoxin [iron-sulfur] cluster Methanosarcina acetivorans DSM 2834
-
 a 2,3-bis-(O-geranylgeranyl)-sn-glycero-phospholipid + 16 reduced ferredoxin [iron-sulfur] cluster + 16 H+
-
 ?
additional information Methanosarcina acetivorans enzyme MA1492 reacts with membrane lipid biosynthetic intermediates, i.e. geranylgeranyl diphosphate (GGPP) and geranylgeranylglyceryl phosphate (GGGP), suggesting that the enzyme also reduces the intermediates ?
-
 ?
additional information Methanosarcina acetivorans DSM 2834 enzyme MA1492 reacts with membrane lipid biosynthetic intermediates, i.e. geranylgeranyl diphosphate (GGPP) and geranylgeranylglyceryl phosphate (GGGP), suggesting that the enzyme also reduces the intermediates ?
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans Q8TQP8
-
-
Methanosarcina acetivorans DSM 2834 Q8TQP8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally poly-His-tagged enzyme MA1492 from Escherichia coli strain KRX by cobalt affinity chromatography Methanosarcina acetivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a 2,3-bis-(O-geranylgeranyl)-sn-glycero-phospholipid + 16 dithionite + 16 H+ i.e. DGGGP-glycerol Methanosarcina acetivorans a 2,3-bis-(O-phytanyl)-sn-glycero-phospholipid + 16 oxidized dithionite
-
 ?
a 2,3-bis-(O-geranylgeranyl)-sn-glycero-phospholipid + 16 dithionite + 16 H+ i.e. DGGGP-glycerol Methanosarcina acetivorans DSM 2834 a 2,3-bis-(O-phytanyl)-sn-glycero-phospholipid + 16 oxidized dithionite
-
 ?
a 2,3-bis-(O-geranylgeranyl)-sn-glycero-phospholipid + 16 reduced ferredoxin [iron-sulfur] cluster + 16 H+ i.e. DGGGP-glycerol Methanosarcina acetivorans a 2,3-bis-(O-phytanyl)-sn-glycero-phospholipid + 16 oxidized ferredoxin [iron-sulfur] cluster
-
 ?
a 2,3-bis-(O-geranylgeranyl)-sn-glycero-phospholipid + 16 reduced ferredoxin [iron-sulfur] cluster + 16 H+ i.e. DGGGP-glycerol Methanosarcina acetivorans DSM 2834 a 2,3-bis-(O-phytanyl)-sn-glycero-phospholipid + 16 oxidized ferredoxin [iron-sulfur] cluster
-
 ?
a 2,3-bis-(O-phytanyl)-sn-glycero-phospholipid + 16 oxidized ferredoxin [iron-sulfur] cluster
-
 Methanosarcina acetivorans a 2,3-bis-(O-geranylgeranyl)-sn-glycero-phospholipid + 16 reduced ferredoxin [iron-sulfur] cluster + 16 H+
-
 ?
a 2,3-bis-(O-phytanyl)-sn-glycero-phospholipid + 16 oxidized ferredoxin [iron-sulfur] cluster
-
 Methanosarcina acetivorans DSM 2834 a 2,3-bis-(O-geranylgeranyl)-sn-glycero-phospholipid + 16 reduced ferredoxin [iron-sulfur] cluster + 16 H+
-
 ?
geranylgeraniol + reduced ferredoxin MA1492 selectively reduces the x-double bond of a geranylgeranyl group Methanosarcina acetivorans 14,15-dihydrogeranylgeraniol + oxidized ferredoxin
-
 ?
additional information enzyme MA1492 reacts with membrane lipid biosynthetic intermediates, i.e. geranylgeranyl diphosphate (GGPP) and geranylgeranylglyceryl phosphate (GGGP), suggesting that the enzyme also reduces the intermediates Methanosarcina acetivorans ?
-
 ?
additional information substrate specificity and regiospecificity of recombinant MA1492, overview. The enzyme is definitely specific to compounds containing C20 geranylgeranyl groups and reduces only one of four double bonds in a geranylgeranyl chain. GC-MS analysis of the product from geranylgeraniol confirms that the reduction selectively occurs on the omega-terminal double bond Methanosarcina acetivorans ?
-
 ?
additional information enzyme MA1492 reacts with membrane lipid biosynthetic intermediates, i.e. geranylgeranyl diphosphate (GGPP) and geranylgeranylglyceryl phosphate (GGGP), suggesting that the enzyme also reduces the intermediates Methanosarcina acetivorans DSM 2834 ?
-
 ?
additional information substrate specificity and regiospecificity of recombinant MA1492, overview. The enzyme is definitely specific to compounds containing C20 geranylgeranyl groups and reduces only one of four double bonds in a geranylgeranyl chain. GC-MS analysis of the product from geranylgeraniol confirms that the reduction selectively occurs on the omega-terminal double bond Methanosarcina acetivorans DSM 2834 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 48300, about, sequence calculation Methanosarcina acetivorans

Synonyms

Synonyms Comment Organism
CrtI homologue
-
 Methanosarcina acetivorans
MA1492
-
 Methanosarcina acetivorans
MA_1492
-
 Methanosarcina acetivorans
phytoene dehydrogenase family protein UniProt Methanosarcina acetivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Methanosarcina acetivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Methanosarcina acetivorans

Cofactor

Cofactor Comment Organism Structure
FAD a short motif is assigned as an FAD binding site Methanosarcina acetivorans
Ferredoxin
-
 Methanosarcina acetivorans
additional information no activity with NAD(P)H, the bound flavin is reduced with dithionite but not with NAD(P)H Methanosarcina acetivorans

General Information

General Information Comment Organism
evolution MA1492 is a distinct type of archaeal geranylgeranyl reductase that is not homologous to known geranylgeranyl reductases, but is homologous to phytoene desaturase CrtI, which is the carotenogenic enzyme that catalyzes oxidation/isomerization of phytoene to lycopene, CrtI, EC 1.3.5.6 Methanosarcina acetivorans
physiological function MA1492 might play a physiological role in the membrane lipid biosynthesis Methanosarcina acetivorans